Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen I
  • Host species
    Rabbit
  • Specificity
    Does not cross-react with Rat Collagen Type III and Rat Elastin.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, RIA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Chicken, Human
  • Immunogen

    Tissue, cells or virus corresponding to Rat Collagen I. Rat collagen Type I extracted and purified from rat skin

  • Positive control
    • IHC-P: Rat skin normal.

Properties

Applications

Our Abpromise guarantee covers the use of ab21287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/80.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RIA 1/200.
ELISA 1/200.

Target

  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • IHC image of Collagen I staining in normal rat skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21287, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Dikina AD  et al. A Modular Strategy to Engineer Complex Tissues and Organs. Adv Sci (Weinh) 5:1700402 (2018). Read more (PubMed: 29876200) »
  • Han WQ  et al. Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition. J Physiol 596:3603-3616 (2018). Read more (PubMed: 29863758) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 25 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (vagina tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
vagina tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 25 2018

Answer

Thank you for contacting us. Standard antigen retrieval techniques are not appropriate for collagen studies because they are usually too harsh, causing strong cell membrane alteration/ disruption and heavy background staining. Below please find a general IHC-P protocol for collagen studies. I hope this helps, please let me know if you need any additional information. --- IHC Protocol for identification of collagens using paraffin-embedded sections. Protocol: Fix tissues in AFA (750ml Alcohol + 20ml Formalin + 50ml Acetic acid + 180ml distilled water) or in Bouin's liquid. Embed in paraffin Section (5 um thick) and place on silanized slides. Unless otherwise noted, all incubations should be done at 20C in a wet chamber Pre-Treatment / Deparaffinization 1. Xylene 10 minutes 2. Xylene-95% alcohol (v/v) 10 minutes 3. 95% alcohol 10 minutes 4. 95% alcohol-distilled water (v/v) 10 minutes 5. Clean with running tap water 30 minutes Treatment 1. Glycine-HCl (100mM) in TBS + Ammonium chloride (50mM) in TBS. Incubate 30 minutes. 2. Rinse with TBS quickly first and then again for 5 minutes. 3. Hyaluronidase (Sigma H3506 type 1-S) 0.2% in TBS. Incubate 15 minutes at 37C. 4. Rinse with TBS quickly first and then again for 5 minutes. Immunolabeling 1. Apply primary specific antibody and control serum (non-immune serum) diluted in TBS containing 3%BSA and 1% Normal goat serum. Incubate overnight. 2. Rinse with TBS + 0.2% Tween 20 quickly first and then again for 10 minutes. 3. Inhibit nonspecific activity with TBS + 3% BSA + 0.9% NaCl. Incubate 20 minutes. 4. Rinse with TBS + 0.2% Tween 20 quickly first and then again for 10 minutes. 5. Inhibit endogenous peroxidase with 10ml TBS + 3% BSA + 500ul 30% hydrogen peroxide + 10mg Sodium azide. Incubate 20 minutes. 6. Rinse with TBS quickly first and then again for 10 minutes. 7. Label using secondary antibody or IHC staining kit. 8. Rinse with TBS quickly first and then again for 10 minutes. 9. Stain with DAB 2-5 minutes. 10. Rinse for 10 minutes with running tap water. 11. Counter stain with Aqueous Harris Hematoxylin (diluted 2/3 in distilled water). Incubate 5 minutes. 12. Rinse for 10 minutes with running tap water. 13. Differentiate with ammonia water (250ml water + 2ml 30% ammonia). Incubate 2 minutes. 14. Section Mounting in aqueous mounting medium.

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