Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, Indirect ELISA, WB, IHC-P, ELISA, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Horse, Cow, Human, Pig, Common marmoset
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen I aa 1-1464. Collagen Type I from human and bovine placenta.
    Database link: P02452

  • Positive control
    • IHC-P: Mouse kidney tissue. Sheep duodenum tissue. Pig lung tissue. Marmoset kidney tissue. Human colon and placenta tissue. Cow small intestine tissue. IHC-Fr: Mouse kidney tissue. Cow tendon tissue. ICC/IF: Horse bronchial fibroblast cells.; Human keloid and normal fibroblasts. WB: Human Collagen Type 1. Wistar rat hepatic stellate cells in GFP-transduced cell lysate.
  • General notes

    ab34710 is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

    For more protocol tips, please seehttps://www.abcam.com/protocols/collagen

     

    It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues.

    Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.8766% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/200.
Indirect ELISA 1/5000 - 1/50000.
WB 1/1000 - 1/10000.

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins here.

Customers have been successful using ab34710 in this application, please see references below (Tillgren V  et al. J Biol Chem 290:918-25; 2015).

IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000 - 1/50000.
ICC/IF 1/500.
IP 1/100.

Target

  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Anti-Collagen I antibody (ab34710) at 1/1000 dilution + Human collagen, 50 ng

    Secondary
    DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT at 1/20000 dilution


    Other Band(s): Collagen Type I splice variants and isoforms.

  • The expression of Collagen I was examined in Normal fibroblasts and keloid fibroblasts using ab34710 at 1/50 dilution in ICC/IF analysis.

    Cells were cultured on microscope coverslips (Thermo Fisher Scientific, Rochester, NY, USA) and treated with NTP (Non-thermal plasma). After 24 h, the slides were washed with PBS, fixed for 20 min in 3.7% formaldehyde and rehydrated in PBS. After blocking for 45 min in bovine serum albumin dissolved in 5% PBS, the slides were incubated for 1 h with sb34710 at 1/50 dilution  then washed with PBS and incubated for 45 min with an Alexa 488-labeled goat anti-rabbit antibody at 1/250 dilution.

    NTP reduced the expression of Type I collagen in KFs as revealed by the immunocytochemistry and Sirus Red stain. Immunofluorescent and Sirus Red staining for Type I collagen were observed in NFs and KFs after treatments with gas or NTP.

    Row 1 and 2: Analysis of Collagen I in Normal fibroblasts by Sirus Red and IF respectively.
    Row 3 and 4: Analysis of Collagen I in Keloid fibroblasts by Sirus Red and IF respectively.

    Column 1 is the control. Column 2 is the control gas treatment (Argon and nitrogen at 200:500). Column 3-5 were exposed to NTP for 10, 30 and 60 seconds respectively.

     

  • ab34710 staining Collagen I in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2.5% horse serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in PBS 1X + 3% of 2.5% horse serum) for 12 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab34710 staining Collagen I in pig lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. A Cy3-conjugated Donkey anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab34710 staining Collagen I in horse bronchial fibroblast cells by Immunocytochemistry. Cells were fixed with acetone and blocking with 3% BSA was performed for 1 hour 30 minutes at 40°C. Samples were incubated with primary antibody (1/500: in 3% BSA/ PBS) for 12 hours at 4°C. An FITC-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/160 as secondary antibody.

  • ab34710 staining Collagen I in sheep jejunum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 0.25% protein block for 1 hour at room temperature; antigen retrieval was by heat mediation in a 10 mM citrate buffer, pH 6. Samples were incubated with primary antibody (1/400) for 1 hour. An Envision +system HRP anti-rabbit, goat polyclonal (HRP polymer conjugation) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of marmoset kidney tissue labeling Collagen I with ab34710 at 1/500 dilution. Tissue samples were fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C. Heat mediated antigen retirval was performed using citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide buffer) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of Collagen I staining in normal human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab34710 at 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab34710 staining Collagen I in cow small intestine tissue sections by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide buffer) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab34710 staining mouse kidney sections (ab4606) by IHC-Fr. Kidney tissue was cryoprotected with 30% sucrose, sectioned using a cryostat at 10 microns and mounted onto slides. After drying overnight in fume hood, sections were fixed in 4% formalin in PBS for 10 minutes. Blocking was performed with 1% BSA for 10 minutes at 21°C. Staining with ab34710 at a 1/100 dilution in TBS/BSA with 0.02% azide was performed for 2 hours at 21°C. A conjugated goat anti-rabbit 594 polyclonal antibody at 1/1000 was used as the secondary antibody.

    See Abreview

  • Detection of collagen I in Wistar rat hepatic stellate cells in GFP-transduced (left lane) and PPAR?-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by native PAGE and electroblotted to nitrocellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS with 5% non-fat milk.

  • Immunohistochemistry of breast carcinoma staining Collagen I with ab34710 at 5μg/ml

  • Immunohistochemical analysis of human colon tissue sections labelling Collagen I with ab34710 at a dilution of 1/200. The sections were fixed with formaldehyde. The secondary antibody used was an HRP conjugated rabbit IgG. Antigen retrieval was heat mediated using CC1.

    See Abreview

  • Immunohistochemical analysis of formaldehyde-fixed frozen bovine tendon sections, labelling Collagen I with ab34710 at a dilution of 1/500 for 2 hours at 21°C. 1% BSA was used as a blocking agent and incubated for 10 minutes at 21°C. Secondary used was a Goat anti-Rabbit Alexa Fluor® 594 conjugate used at 1/1,000.

    See Abreview

References

This product has been referenced in:
  • Peng J  et al. YAP and TAZ mediate steroid-induced alterations in the trabecular meshwork cytoskeleton in human trabecular meshwork cells. Int J Mol Med 41:164-172 (2018). Read more (PubMed: 29115373) »
  • Zheng L  et al. MiR-26a-5p regulates cardiac fibroblasts collagen expression by targeting ULK1. Sci Rep 8:2104 (2018). WB . Read more (PubMed: 29391491) »
See all 433 Publications for this product

Customer reviews and Q&As

1-10 of 73 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Intestine
Blocking step
(agent) for 4 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde

Dr. Li Li

Verified customer

Submitted Sep 19 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblast)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
10 µg
Specification
Fibroblast
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2018

Application
Western blot
Sample
Salmo salar Tissue lysate - whole (Skin)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
10 µg
Treatment
500 mM acetic acid
Specification
Skin
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 20 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
lymph node
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 20% · Temperature: 37°C
Fixative
Formaldehyde

Ms. Maria Daskalaki

Verified customer

Submitted Jun 06 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular Smooth Muscle Cell)
Permeabilization
Yes - NP-40
Specification
Vascular Smooth Muscle Cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 17 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung fibroblast)
Permeabilization
No
Specification
lung fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - PBS 0.1%tween
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Mrs. Aline Chrétien

Verified customer

Submitted Feb 27 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Permeabilization
Yes - Triton X-100
Specification
Bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Jan 15 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
Permeabilization
No
Specification
Skin
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 29 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Jaculus jaculus Tissue sections (Muscle)
Permeabilization
Yes - 0.3% triton
Specification
Muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Mai Tran

Verified customer

Submitted Dec 20 2017

1-10 of 73 Abreviews or Q&A

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