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    collagen-i-antibody-ab34710.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen I antibody (ab34710)

  • Datasheet
  • SDS
Reviews (90)Q&A (11)References (1342)

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Western blot - Anti-Collagen I antibody (ab34710)

    Key features and details

    • Rabbit polyclonal to Collagen I
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Collagen I antibody [EPR22894-89] (ab260043)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Collagen I antibody
      See all Collagen I primary antibodies
    • Description

      Rabbit polyclonal to Collagen I
    • Host species

      Rabbit
    • Specificity

      This product is not recommended for use under denaturing conditions in WB. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins here.

      ab34710 has <5% cross-reactivity with Collagen III

      Customers have been successful using ab34710 in this application, please see references below (Tillgren V  et al. J Biol Chem 290:918-25; 2015).

      Positive Control: Human stomach, skin and adrenal gland tissue lysates.

    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length native protein (purified) corresponding to Human Collagen I aa 1-1464. Collagen Type I from human and bovine placenta.
      Database link: P02452

    • Positive control

      • WB: human collagen.
    • General notes

      Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

      This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen protein during your experiment.

      PLEASE READ THESE IMPORTANT PROTOCOL TIPS, click here for the english version or here for the mandarin version. 

      It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.8766% Sodium chloride, 0.42% Potassium phosphate
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab34710 has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Collagen
      • Stem Cells
      • Mesenchymal Stem Cells
      • Osteogenesis

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Collagen I protein (ab158152)
    • Related Products

      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      • Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
      • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
      • Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216791)
      • Native Cow Collagen I protein (ab7526)
      • Native Human Collagen I protein (ab7533)
      • Anti-Laminin 5 antibody [P3H9-2] (ab78286)
      • Anti-MMP2 antibody [6E3F8] (ab86607)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34710 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (10)
    1/1000 - 1/10000.
    Notes
    WB
    1/1000 - 1/10000.

    Target

    • Function

      Type I collagen is a member of group I collagen (fibrillar forming collagen).
    • Tissue specificity

      Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
    • Involvement in disease

      Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
      Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
      Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
      Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
      Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
    • Sequence similarities

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications

      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P02452 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1277 Human
      • Entrez Gene: 1278 Human
      • Omim: 120150 Human
      • Omim: 120160 Human
      • SwissProt: P02452 Human
      • SwissProt: P08123 Human
      • Unigene: 172928 Human
      • Unigene: 681002 Human
      • Alternative names

        • Alpha 1 type I collagen antibody
        • Alpha 2 type I collagen antibody
        • alpha 2 type I procollagen antibody
        • alpha 2(I) procollagen antibody
        • alpha 2(I)-collagen antibody
        • Alpha-1 type I collagen antibody
        • alpha1(I) procollagen antibody
        • CO1A1_HUMAN antibody
        • COL1A1 antibody
        • COL1A2 antibody
        • collagen 1 antibody
        • collagen alpha 1 chain type I antibody
        • Collagen alpha-1(I) chain antibody
        • collagen alpha-1(I) chain preproprotein antibody
        • Collagen I alpha 1 polypeptide antibody
        • Collagen I alpha 2 polypeptide antibody
        • collagen of skin, tendon and bone, alpha-1 chain antibody
        • collagen of skin, tendon and bone, alpha-2 chain antibody
        • Collagen type I alpha 1 antibody
        • Collagen type I alpha 2 antibody
        • EDSC antibody
        • OI1 antibody
        • OI2 antibody
        • OI3 antibody
        • OI4 antibody
        • pro-alpha-1 collagen type 1 antibody
        • type I proalpha 1 antibody
        • type I procollagen alpha 1 chain antibody
        • Type I procollagen antibody
        see all

      Images

      • Western blot - Anti-Collagen I antibody (ab34710)
        Western blot - Anti-Collagen I antibody (ab34710)
        Anti-Collagen I antibody (ab34710) at 1/1000 dilution + Human collagen, 50 ng

        Secondary
        DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.


        DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.

        Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

        Other Band(s): Collagen Type I splice variants and isoforms.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1342)

      Publishing research using ab34710? Please let us know so that we can cite the reference in this datasheet.

      ab34710 has been referenced in 1342 publications.

      • Zhang D  et al. CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin a5 expression. Cell Death Differ 28:283-302 (2021). PubMed: 32814879
      • Stantzou A  et al. Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse plantaris muscle. Neuropathol Appl Neurobiol 47:218-235 (2021). PubMed: 32772401
      • Liao Y  et al. Therapeutic silencing miR-146b-5p improves cardiac remodeling in a porcine model of myocardial infarction by modulating the wound reparative phenotype. Protein Cell 12:194-212 (2021). PubMed: 32845445
      • Song J  et al. Myeloid interleukin-4 receptor a is essential in postmyocardial infarction healing by regulating inflammation and fibrotic remodeling. Am J Physiol Heart Circ Physiol 320:H323-H337 (2021). PubMed: 33164548
      • Yang L  et al. Bioactive Sr2+/Fe3+co-substituted hydroxyapatite in cryogenically 3D printed porous scaffolds for bone tissue engineering. Biofabrication 13:N/A (2021). PubMed: 33260162
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      1-10 of 11 Q&A

      Question

      I currently have ab300 and ab34710 which are meant to be stored in the -20 freezer once aliquotted. However, our freezer recently broke and the antibodies were at +10 for approximately 12 hours. Do you think that these products were ruined during that thaw time? Do you have any data on samples that are stored unfrozen for an extended period of time?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 05 2012

      Answer

      I think the antibodies should be fine if they were only at +10C for that short a period of time. Most of our antibodies will remain stable at room temperature for up to 7 days without any negative effect.

      Read More

      Abcam Scientific Support

      Answered on Oct 05 2012

      Question

      I have just purchased the Anti Collagen I antibody (#ab34710) from a Brazilian representative (Biogen) and I would like to know which is the volume of antibody per vial, once the intention is to use it to analyze about 270 immunoslides (applying a dillution of 1:200), and I need to know how many vials I would have to acquire.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 03 2012

      Answer

      As the antibody is delivered at 100 µg at 1mg/ml, this means that you should have got 100µl. Regarding your question of how many vials you should orderI have to say that I do not have a definite answer: this will depend on how much working solution per slide you will use (1:200 of 50µl or 100µl, or more or less) as this will depend on your slides size.

      Read More

      Abcam Scientific Support

      Answered on Oct 03 2012

      Question

      What is the molecular weight of collagen I?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 09 2012

      Answer


      Collagen I forms trimers of one alpha 2(I) and two alpha 1(I) chains have a predicted molecular weight of 139kDa and 130kDa respectivley, based on the data available from Uniprot.

      http://www.uniprot.org/uniprot/P02452

      http://www.uniprot.org/uniprot/P08123

      Abreviews placed by customers using this product have shown the bands on western blot to be between 120kDa and 160kDA. I have added links to these reviews below:

      https://www.abcam.com/Collagen-I-antibody-ab34710.html&tab=abreviews&intabreviewid=30165

      https://www.abcam.com/Collagen-I-antibody-ab34710.html&tab=abreviews&intabreviewid=25145

      https://www.abcam.com/Collagen-I-antibody-ab34710.html&tab=abreviews&intabreviewid=23838

      Read More

      Abcam Scientific Support

      Answered on Aug 09 2012

      Question

      Another expert at our institution acquainted with your antibodies suggested " ab21286, ab34710, and ab292 (however this could cross-react with other collagens) ". Before ordering the material I wanted to check with you. Which one do you suggest that I should buy?

      Read More

      Abcam community

      Verified customer

      Asked on May 23 2012

      Answer

      Each of these 3 antibodies looks like it would be useful for your experiment. We will guarantee all of them to perform as described on the online datasheet. I think ab34710 looks like a good antibody cited in numerous publications that appears specific for collagen 1.

      Read More

      Abcam Scientific Support

      Answered on May 23 2012

      Question

      I recently purchased an anti-collagen I (ab34710) and an anti-collagen IV antibody (ab6586) from you, and managed to get them to work successfully in IHC.
      I am now hoping to do a Western blot on cultured cells, however I have never done Westerns before. Reading your Western Blotting for beginners guide I have seen that I need to use a non-denaturing and non-reducing conditions. Do you have a specific protocol for this, as the beginners protocol is a bit confusing as to where I should be doing things differently? Or do you know of any references where they have used these antibodies on cultured cells and media?

      Read More

      Abcam community

      Verified customer

      Asked on May 07 2012

      Answer

      Indeed, we recommend native (non-denaturing, non-dissociating) PAGE and western blotting when using this product. These are obtained by omitting SDS and the reducing agentsin the migration buffer as well as in the sample buffer. Please find a link with detailed recommendations for the buffer preparations:

      http://www.molecularinfo.com/MTM/G/G1/G1-1.html
      Below are two links that provide references (and protocols) for the use of this product in western blotting. Although we recommend native conditions (the epitope depends on a 3-dimensional structure) one of these references did have success using reduced conditions.

      http://www.jbc.org/cgi/content/full/277/20/18229
      http://www.jbc.org/cgi/content/full/279/12/11392

      I can also recommend this general reference:
      Hames BD. Gel Electrophoresis of Proteins: A Practical Approach. 3rd ed. New
      York: Oxford University Press: 1998

      Read More

      Abcam Scientific Support

      Answered on May 07 2012

      Question

      I want to purchase anti-collagen type 1 to be used in IHC and ICC/IF experiments on human corneal sections. Can you please suggest one of your antibodies?

      Read More

      Abcam community

      Verified customer

      Asked on May 02 2012

      Answer

      Any of the following antibodies would work for your experiment.

      Rabbit poly, ab292 and ab34710

      Mouse mono; ab90395

      Read More

      Abcam Scientific Support

      Answered on May 02 2012

      Question

      The Western blot has a band at 110 kda and another at 200 kDa (when using ab34710).
      Any ideas as to why the extra bands appeared on the blot?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 28 2012

      Answer

      The Collagen I molecule undergo extracellular processing of the procollagen molecule to remove the propeptide sequences at each end of the molecule. In COLA2A1 there are 2 propeptides one is at N-terminal amino acid 25-75 and at C-terminus aa1103 to 1366. The 110 kDa is molecular weight of processed collagen monomer and 200 kDa for dimer.

      http://www.uniprot.org/uniprot/P08123

      Same is true for COL1A1 http://www.uniprot.org/uniprot/P02452

      Read More

      Abcam Scientific Support

      Answered on Mar 28 2012

      Question

      Can I boil the protein sample for Collagen I Western Blot before loading the sample?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 12 2011

      Answer

      No, you should not boil the samples before loading into the gel.

      Read More

      Abcam Scientific Support

      Answered on Dec 12 2011

      Question

      We have recently received a consignment of antibodies from you on Friday. Unfortunately, I was not informed of this delivery until today and therefore the antibodies have been left at room temperature over the weekend. I have now placed the antibodies immediately into 4C for storage but am wondering if these antibodies would still be viable after being left at room temperature all this time. These antibodies are unconjugated antibodies (both monoclonal and polyclonal).

      Read More

      Abcam community

      Verified customer

      Asked on Oct 04 2011

      Answer

      We recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity.

      Read More

      Abcam Scientific Support

      Answered on Oct 04 2011

      Question

      Which mammalian species does ab34710 react with?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 18 2007

      Answer

      ab34710 reacts with human, bovine, rat, and mouse.

      Read More

      Abcam Scientific Support

      Answered on Jan 22 2007

      1-10 of 11 Q&A

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