Non-Abcam antibody was used: anti Rabbit IgG Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 594
Fresh frozen tissues were cut at 10 microns, then mounted onto Superfrost Plus slides. After drying overnight in a working Fume hood, sections were fixed in 4% Formalin in PBS for 10 mins, before immunostaining. It seems to identify small fibres very well. However, I would have liked to have been able to use a lower dilution factor. NB: by using a biotinylated secondary and then incubating in avidin-Fluorochrome, you will be able to dilute the primary Ab to at least 1/200.
Mr. Carl Hobbs
Submitted Feb 02 2012
Get resources and offers direct to your inboxSign up