Immunohistochemistry (Frozen sections) abreview for Anti-Collagen I antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Cow Tissue sections (Tendon)
Permeabilization
No
Specification
Tendon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Submitted image shows three slightly separated fascia of tendons ( not good quality tissue). There is a strong diffuse positivity throughout the tendons and their various sheaths. Contrast this image with that of my image for abreview of ab108930 ( anti Tenascin C) which is of a directly sequential section. I have muted the positivity in the tendon fibres because of the intensity of the epi,peri and endo tendineums.

Mr. Carl Hobbs

Verified customer

Submitted Oct 29 2015

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