All incubation @ RMT. automated IHC:Dako autostainer plus used. wash buffer used TBS 1x tween20 (0.05% tween-20). step for enzyme block for 10minutes with Dako Dual endogenous enzyme block. En vision HRP labelled polymer de Dako (anti-Rabbit) incubation for 30min. couter-stain with mayer s hemaroxilyn for 1 min.
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Submitted Jul 14 2017
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