Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
No
Specification
jejunum
Blocking step
protein block-Dako as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: RMT°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: antibody diluent-Dako
Dilution
1/400

Secondary antibody

Name
Non-Abcam antibody was used: EnVision +system HRP Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
All incubation @ RMT. automated IHC:Dako autostainer plus used. wash buffer used TBS 1x tween20 (0.05% tween-20). step for enzyme block for 10minutes with Dako Dual endogenous enzyme block. En vision HRP labelled polymer de Dako (anti-Rabbit) incubation for 30min. couter-stain with mayer s hemaroxilyn for 1 min.

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Submitted Jul 14 2017

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