ad TREATMENT) Cooked skin (gelatin) dissolved in 500 mM acetic acid. Dialyzed against water after separation from unsoluable components. (Gelatin was treated with acetic acid to solubilize it)
ad BLOCKING AGENT) 1 hour treatment with PBS-T (no BSA or else)
ad BLOCKING TEMPERATURE) Room temperature: 22°C
ad BANDS) bands at 136 kDa and 127 kDa refer to the collagen type-I alpha-1 chain and alpha-2 chain (mass spectrometry analysis). Bands above 180 kDa may represent oligomeric collagen type-I.
ad POSITIVE CONTROL) MCF-7 lysate was planned as positive control, but the lysate appeared as smear on the gel. Thus, the positive control was left.
ad IMAGE) all samples: Cooked skin (gelatin) from Salmo salar
1) gelatin, estimated amount: ~10 µg (prim ab: ab34710)
2) extract, 10 µg, treated with 500 mM acetic acid (prim ab: ab34710)
3) gelatin, estimated amount: ~10 µg (negative control)
4) extract, 10 µg, treated with 500 mM acetic acid 10 µg (negative control)
lanes 1 and 3: loaded with ~10 µg protein (gelatin directly loaded onto gel)
Abcam user community
Submitted Jun 20 2018
Get resources and offers direct to your inboxSign up