Western blot abreview for Anti-Collagen I antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblast)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
10 µg
Specification
Fibroblast
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 130 kDa

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Submitted Jun 21 2018

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