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    collagen-i-antibody-col-1-ab6308.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-Collagen I antibody [COL-1] (ab6308)

  • Datasheet
  • SDS
Reviews (27)Q&A (10)References (283)

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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)
  • Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
  • Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
  • Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)

Key features and details

  • Mouse monoclonal [COL-1] to Collagen I
  • Suitable for: IHC-Fr, WB
  • Reacts with: Human
  • Isotype: IgG1

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Recombinant Human Collagen I protein (ab158152)

View more associated products

Overview

  • Product name

    Anti-Collagen I antibody [COL-1]
    See all Collagen I primary antibodies
  • Description

    Mouse monoclonal [COL-1] to Collagen I
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-Fr
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Cow Collagen I. Cow skin collagen type I

  • Epitope

    The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.
  • Positive control

    • WB: Natural Cow Collagen I protein (ab7526), IHC-Fr: Human tonsil tissue.
  • General notes

    Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.0976% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    COL-1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Native Cow Collagen I protein (ab7526)
    • Native Human Collagen I protein (ab7533)
  • Recombinant Protein

    • Recombinant Human Collagen I protein (ab158152)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
    • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
    • Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216791)
    • 10x Citrate Buffer pH 6.0 (ab64214)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-Fr
Human
WB
Human
All applications
Rat
Cow
Pig
Deer
Application Abreviews Notes
IHC-Fr (1)
Use a concentration of 3.5 - 7 µg/ml.

(amplification required).Use on unfixed tissue or acetone fixed tissue.

WB (2)
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Notes
IHC-Fr
Use a concentration of 3.5 - 7 µg/ml.

(amplification required).Use on unfixed tissue or acetone fixed tissue.

WB
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease

    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282187 Cow
    • Entrez Gene: 282188 Cow
    • Entrez Gene: 1277 Human
    • Entrez Gene: 1278 Human
    • Entrez Gene: 29393 Rat
    • Entrez Gene: 84352 Rat
    • Omim: 120150 Human
    • Omim: 120160 Human
    • SwissProt: P02453 Cow
    • SwissProt: P02465 Cow
    • SwissProt: P02452 Human
    • SwissProt: P08123 Human
    • SwissProt: P02454 Rat
    • SwissProt: P02466 Rat
    • Unigene: 172928 Human
    • Unigene: 489142 Human
    • Unigene: 681002 Human
    • Unigene: 107239 Rat
    • Unigene: 2953 Rat
    see all
  • Alternative names

    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)
    Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi

    Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.

  • Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
    Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
    Lane 1 : Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/ml
    Lane 2 : Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/ml
    Lane 3 : Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/ml
    Lane 4 : Anti-Collagen I antibody [COL-1] (ab6308) at 0 µg/ml

    All lanes : Recombinant human Collagen 1

    Predicted band size: 130 kDa

  • Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
    Western blot - Anti-Collagen I antibody [COL-1] (ab6308)This image is courtesy of an anonymous Abreview
    Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg

    Secondary
    An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 130 kDa


    Exposure time: 24 hours

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)
    Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)

    Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (283)

    Publishing research using ab6308? Please let us know so that we can cite the reference in this datasheet.

    ab6308 has been referenced in 283 publications.

    • Han L  et al. Protective mechanism of SIRT1 on Hcy-induced atrial fibrosis mediated by TRPC3. J Cell Mol Med 24:488-510 (2020). PubMed: 31680473
    • Chen S  et al. Evaluation of BMP-2 and VEGF loaded 3D printed hydroxyapatite composite scaffolds with enhanced osteogenic capacity in vitro and in vivo. Mater Sci Eng C Mater Biol Appl 112:110893 (2020). PubMed: 32409051
    • Zheng XY  et al. Compound LM9, a novel MyD88 inhibitor, efficiently mitigates inflammatory responses and fibrosis in obesity-induced cardiomyopathy. Acta Pharmacol Sin N/A:N/A (2020). PubMed: 32341464
    • Huang H  et al. The MicroRNA MiR-29c Alleviates Renal Fibrosis via TPM1-Mediated Suppression of the Wnt/ß-Catenin Pathway. Front Physiol 11:331 (2020). PubMed: 32346368
    • Shao S  et al. Lysyl hydroxylase 3 increases collagen deposition and promotes pulmonary fibrosis by activating TGFß1/Smad3 and Wnt/ß-catenin pathways. Arch Med Sci 16:436-445 (2020). PubMed: 32190155
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 37 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [COL-1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (nasal epithelium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH6
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    nasal epithelium
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Catherine Heyward

    Verified customer

    Submitted Dec 15 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [COL-1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen I antibody [COL-1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (NHDF)
    Permeabilization
    Yes - triton
    Specification
    NHDF
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 05 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Collagen I antibody [COL-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Collagen I antibody [COL-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TrintonX100
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2020

    Immunocytochemistry abreview for Anti-Collagen I antibody [COL-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Dog Cultured Cells (Dermal fibroblast)
    Permeabilization
    Yes - 0.1%TritonX100
    Specification
    Dermal fibroblast
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 05 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Collagen I antibody [COL-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Collagen I antibody [COL-1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 20 2020

    ELISA abreview for Anti-Collagen I antibody [COL-1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Natural collagen protein I ab7533 and cell culture)
    Specification
    Natural collagen protein I ab7533 and cell culture
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Type
    Sandwich (Capture)
    Read More

    Abcam user community

    Verified customer

    Submitted May 26 2017

    Western blot abreview for Anti-Collagen I antibody [COL-1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (skin)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (gel 10%)
    Loading amount
    20 µg
    Specification
    skin
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 10 2016

    1-10 of 37 Abreviews or Q&A

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