Overview

  • Product name
    Anti-Collagen I antibody [COL-1]
    See all Collagen I primary antibodies
  • Description
    Mouse monoclonal [COL-1] to Collagen I
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, Dot blot, ICC/IF, Electron Microscopy, Indirect ELISA, ELISA, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Human, Pig, Monkey, Rhesus monkey, Deer
  • Immunogen

    Full length native protein (purified) corresponding to Cow Collagen I.

  • Epitope
    The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.
  • Positive control
    • WB: Natural Cow Collagen I protein (ab7526), total pig skin lysate, human kidney lysate (see reviews)
  • General notes

    Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab6308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 3.5 - 7 µg/ml.

(amplification required).Use on unfixed tissue or acetone fixed tissue.

Dot blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17230415
Electron Microscopy Use at an assay dependent concentration. PubMed: 17016762
Indirect ELISA Use at an assay dependent concentration.
IF Use at an assay dependent concentration. PubMed: 28135282
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.

IHC-FoFr Use at an assay dependent concentration. PubMed: 17016762

Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.

Target

  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.

  • ab6308 at a 1/1000 dilution staining in rabbit polymer scaffold with stem cells by Immunocytochemistry/ Immunofluorecence, incubated for 2 hours at 20°C. PFA fixed. Permeabilized using 0.5% Triton X-100. Blocked with 3% BSA for 2 hours at 4°C. Secondary used at 1/1000 dilution monoclonal donkey anti-mouse IgG (H+L) conjugated to FITC.1st column: DAPI stain (blue)2nd column: collagen type I (green)3rd column: merge image

    See Abreview

  • Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg

    Secondary
    An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 130 kDa


    Exposure time: 24 hours

    See Abreview

References

This product has been referenced in:
  • Windhövel C  et al. Comparison of Six Different Silicones In Vitro for Application as Glaucoma Drainage Device. Materials (Basel) 11:N/A (2018). ICC/IF ; Human . Read more (PubMed: 29495462) »
  • Min JJ  et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). Read more (PubMed: 29682576) »
See all 149 Publications for this product

Customer reviews and Q&As

1-10 of 29 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (Natural collagen protein I ab7533 and cell culture)
Specification
Natural collagen protein I ab7533 and cell culture
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted May 26 2017

Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
20 µg
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Question
Answer

Unfortunately we do not routinely determine the affinity constant for our antibodies and therefore we would not have this information to share we you.

We do however have an alternative anti-collagen 1 antibody for which we have determined the dissociation constant to be 1.22 x 10-10 M. This is for antibody Anti-Collagen I antibody [EPR7785] (ab138492).

Alternatively, you may be able to determine the Kd by methods such as those described in Rath et al, 1988 (PMID 3276795), or Friguet et al, 1985 (PMID 3981007).

Read More

Answer

It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on non-denatured three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen.
The triple-helical region of collagen 1 corresponds to amino acids 179 – 1192. If you want to use denaturing and reducing conditions, you will need to find an antibody which recognises a region outside the triple-helical region so either an antibody recognising the non helical region N-terminal (aa 162 – 178) or C-terminal (aa 1193 – 1218).
The only antibody which would be suitable is Anti-Collagen I antibody (ab84956) which is guaranteed to work in mouse and rat in WB.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Lung Fibroblasts)
Specification
Lung Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Type
Other

Abcam user community

Verified customer

Submitted Jan 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Nasal polyp, tonsil, bronchial biopsies)
Specification
Nasal polyp, tonsil, bronchial biopsies

Abcam user community

Verified customer

Submitted Aug 24 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Rabbit joint tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Innovex Biosciences Unitrieve
Specification
Rabbit joint tissue
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 14 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Cercopithecus neglectus - Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Cercopithecus neglectus - Skin
Fixative
FineFix

Abcam user community

Verified customer

Submitted Apr 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Otic area)
Specification
Otic area
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 50 minute(s) · Concentration: 20% · Temperature: 19°C

Abcam user community

Verified customer

Submitted Feb 17 2010

1-10 of 29 Abreviews or Q&A

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