Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [EPR7785]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tuberculosis samples)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - PBS with 3% triton
Specification
Tuberculosis samples
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.025% Triton
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti Rabbit BD
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Jul 27 2015

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