Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [EPR7785]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Articular cartilage + chondrocytes)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Bovine testicular hyaluronidase
Permeabilization
No
Specification
Articular cartilage + chondrocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 10% goat serum + 3% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Dako Labelled Polymer-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Inactivated endogenous peroxidase by using Bloxall. As expected, the uploaded image shows the negative staining of pig articular cartilage on the left and the positive staining of collagen gel encapsulating pig chondrocytes on the right.

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Submitted Aug 07 2015

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