Thank you for your review of this antibody's performance with a previously untested species (mouse) and in a previously untested application (IHC of frozen tissue). For formalin-fixed, paraffin-embedded tissue, we found that a heat-mediated antigen retrieval before incubation with the antibody improved signal intensity. This could also be applied to sections of frozen tissue if they are not too fragile.
Dr. Sueli Marques
Submitted Dec 22 2015
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