Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citraconic anhydride
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/3000
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti rabbit 555
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Aug 01 2017