Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (nasal turbinates)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer, pH 6
Permeabilization
Yes - 0.1% Triton X-100
Specification
nasal turbinates
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% normal goat serum in PBS
Dilution
1/300
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti-rabbit AlexaFluo 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Tested mouse, rat and pig tissues for IHC-P. Antibody worked well for pig but not for mouse or rat (see related reviews)
Abcam response
Abcam has not tested ab138492 in Rat samples and does not know if the antibody should have worked.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Catherine Heyward
Verified customer
Submitted Sep 15 2021