• Product name
    Anti-Collagen I + Collagen III antibody
    See all Collagen I + Collagen III primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + Collagen III
  • Host species
  • Specificity
    Specificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining.
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Full length native collagen type I and III from pig skin.

  • Positive control
    • Pig kidney and skin. Cultured porcine cells.


  • Form
    Lyophilised:Reconstitute with 1ml of distilled water and add preservative if required.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Pooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab24135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10 - 1/20.


  • Relevance
    Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
  • Cellular localization
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • COL3A1 antibody
    • Collagen fetal antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • Collagen type III alpha 1 antibody
    • OI4 antibody
    • Type I collagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all


ab24135 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


ab24135 is unlikely to be a good choice for this purpose, though it is raised against pig collagen. It will be difficult to find anti-collagen antibodies that are reactive with one species and not another, given that collagens are well-conserved between rat and pig. Rat reactivity is not listed for ab24135 because it has not been tested against rat samples, but in general, polyclonal anti-collagen antibodies are reactive with a broad range of species.

If your collagen is expected to contain the procollagen region (intracellular, not secreted), then you should consider ab64409. Although reactivity with rat collagen has not been assessed, there is less conservation in this region. However, ab64409 is raised in rat, so a secondary antibody against rat IgG may react with endogenous rat IgG, if your samples are rat tissue.

Read More


Sign up