Overview

  • Product name
    Anti-Collagen III antibody [1E7-D7/Col3]
    See all Collagen III primary antibodies
  • Description
    Mouse monoclonal [1E7-D7/Col3] to Collagen III
  • Host species
    Mouse
  • Specificity
    ab23445 is highly specific for type III Collagen. It has been shown to have no cross reactivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • Tested applications
    Suitable for: ELISA, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Pig, Kangaroo
    Does not react with: Sheep, Cow
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen III.
    Database link: P02461

  • Epitope
    The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.
  • Positive control
    • Frozen and unfixed human or kangaroo skin tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab23445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500.
IHC-Fr Use at an assay dependent concentration. Note: Usually ab23445 is used in unfixed tissue slides. If fixation of tissue is required, acetone or ethanol is recommended.
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in disease
    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type III collagen antibody
    • Alpha1 (III) collagen antibody
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha 1 chain antibody
    • Collagen type III alpha antibody
    • Collagen, fetal antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Images

  • Immunohistochemical analysis of frozen pig skin tissue, staining Collagen III with ab23445.

    Tissue was fixed with acetone and blocked with 10% serum for 10 minutes 20°C. Samples were incubated with primary antibody (1/500 in TBS, 0.02% Triton x-100, 2% BSA) for 16 hours at 4°C. An undiluted biotin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Paraffin-embedded sections) analysis of human bronchus tissue labelling Collagen III with ab23445.

References

This product has been referenced in:
  • Kishi Y  et al. Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium. PLoS One 12:e0189522 (2017). IHC-P ; Human . Read more (PubMed: 29253010) »
  • Hill DS  et al. A Novel Fully Humanized 3D Skin Equivalent to Model Early Melanoma Invasion. Mol Cancer Ther 14:2665-73 (2015). IHC-P ; Human . Read more (PubMed: 26330548) »
See all 6 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
burn wound with stem cell treatment
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample
Human Tissue sections (huam whole fat tissue)
Specification
huam whole fat tissue
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (pig skin frozen sections)
Specification
pig skin frozen sections
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 24 2013

Question

Thank you for your detailed answer about the anti-collagen III antibody ab6310.

That's a pity indeed that the validity on pig was initially a mistake but these things happen. I am a regular customer who appreciate very much your policy about customer feedback and reviewing, and I'll submit an abreview about IHC-Fr in pig skin after performing the few more tests discussed.

In return, to answer your questions:

- From what I've read and optimized so far on frozen sections with different kind of antibodies, the antigen retrieval step is usually not needed as the fixation time is very short.

I don't have any pronase, and I'm not sure it's worth for me buying some only for a test, with no insurance the antibody will work. I can try fixing in acetone instead or formalin see if it changes something.

- I will follow your advice and block with only donkey serum or BSA but not both at the same time

- The secondary antibody works well, I've written reviews about ab90395 (collagen I) and ab6328 (fibronectin EDA), where I used the same anti-mouse Cy2 secondary antibody on similar pig skin frozen sections with good results.

- I have performed the procedure on a couple of separate samples, and I did it twice on independent batches before contacting the technical service, to ensure the problem was from the antibody and not from my experimental procedure.

I’d like to try the reference ab23445, as I also would like to do some western blotting. If this second one doesn't work, will I still be eligible to try the other one, or for a refund?

Thank you very much

Best regards

Read More
Answer

Thank you foryour understanding and cooperation. The details provided enable us to closely monitor the quality of our products.

I hope the suggestions may proove useful with ab6310. It may be worth trying citrate antigen retreival with the PFA fixationif you have the reagents. It is unusual to use antigen retreival with IHC-Fr, however laboratory tests do indicate that this can sometimes improve the results.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedthe alternativefree of charge replacement of ab23445with the order number 1132685.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. The ab23445 is tested and covered by the guarantee for IHC-Fr, WB and pig samples. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, die für Ihre Versuchsvorhaben geeignet sein könnten:

ab23446:mouse monoclonalanti-Collagen I antibody, reacts with pig, does not react with mouse. Daherbesteht die Möglichkeit, dass er nicht mit Ratte kreuzreagiert. Bisher wurde dies nicht getestet.

ab88147: Mouse monoclonal Anti-Collagen I antibody, reacts with human andmouse. Sequenzähnlichkeit des Immunogens gegenüber Schwein: 77%, gegenüber Ratte: 96%. Es könnte also sein, dass er mit Ratte kreuzreagiert, aber nicht mit Schwein.

ab82354: Mouse monoclonalAnti-Collagen III antibody, reacts with rat. Aufgrund einer Sequenzähnlichkeit des humanen Kollagen III von weniger als 57% gegen das Ortholog des Schweins ist die Wahrscheinlichkeit einer Kreuzreaktivität sehr gering.

ab78032: Mouse monoclonal Anti-Collagen III antibody, reacts with human. Aufgrund einer Sequenzähnlichkeit des humanen Kollagen III von weniger als 57% gegen das Ortholog des Schweins ist die Wahrscheinlichkeit einer Kreuzreaktivität sehr gering. Die Sequenzaehnlichkeit gegen Ratte liegt bei 90%; er könnte also mit Ratte funktionieren.

Für den von Ihnen genannten ab23445 hatte ich im Labor nachgefragt, das uns bestätigt hat, dass dieser Antikörper leider nicht zwischen dem Kollagen III des Schweins bzw. der Ratte diskriminieren kann. Ich habe das Datenblatt entsprechend aktualisiert.

Einige der Antikörper haben wir bisher nicht an Paraffinschnitten (ICH-P) getestet, aber wir sehen keinen Grund, warum sie nicht für diese Anwendung geeignet sein sollten.

Mit jedem deroben genannten Antikörper können Sie an unserem 100%igen Abreview-Rabattprogramm teilnehmen: Wenn Sie den Antikörper selbst testen möchten, bekommen Sie einen beliebigen primären Antikörper kostenlos von uns, wenn Sie uns ein Abreview mit dem Testresultat zusenden.Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Gerne stehe ich auch für weitere Fragen zur Verfügung.

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up