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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen III antibody (ab7778)

  • Datasheet
  • SDS
Reviews (18)Q&A (25)References (331)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Western blot - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

Key features and details

  • Rabbit polyclonal to Collagen III
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Collagen III antibody [EPR17673] (ab184993)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Collagen III antibody
    See all Collagen III primary antibodies
  • Description

    Rabbit polyclonal to Collagen III
  • Host species

    Rabbit
  • Specificity

    This type specific collagen antibody only recognizes 3D epitopes. Negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Collagen III aa 1-1466.
    Database link: P02461

  • Positive control

    • Natural Cow Collagen III protein (ab7528) can be used as a positive control in WB. Human skin. Human testicle tissue.
  • General notes

    For more protocol tips, please see: https://www.abcam.com/protocols/collagen

    At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.8766% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Native Cow Collagen III protein (ab7528)
    • Native Human Collagen III protein (ab7535)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • 10x Citrate Buffer pH 6.0 (ab64214)
    • Anti-MMP2 antibody [6E3F8] (ab86607)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Mammals
Application Abreviews Notes
IHC-P (10)
1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
1/5000 - 1/10000. Predicted molecular weight: 138 kDa.
Notes
IHC-P
1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/5000 - 1/10000. Predicted molecular weight: 138 kDa.

Target

  • Function

    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in disease

    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02461 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1281 Human
    • Entrez Gene: 12825 Mouse
    • Entrez Gene: 84032 Rat
    • Omim: 120180 Human
    • SwissProt: P02461 Human
    • SwissProt: P08121 Mouse
    • SwissProt: P13941 Rat
    • Unigene: 443625 Human
    • Unigene: 249555 Mouse
    • Unigene: 3247 Rat
    see all
  • Alternative names

    • Alpha 1 type III collagen antibody
    • Alpha1 (III) collagen antibody
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha 1 chain antibody
    • Collagen type III alpha antibody
    • Collagen, fetal antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Xiao, H. et al PLoS One. 2013 Apr 9;8(4):e60173. doi: 10.1371/journal.pone.0060173. Print 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    A2AR activity affected UUO (unilateral ureteral obstruction)-induced deposition of collagen III

    (A) Representative immunohistochemistry of Collagen III) (Col III) from the A2AR KO and WT mice, at day 3, 7 and 14 post-UUO or Sham, following treatment of CGS21680 (CGS) or vehicle (Veh). Scale bar  = 50 µm, 400×. 

    Collagen III in rat kidney tissue is detected ab7778 at 1/400 dilution. Pressure cooker mediated antigen retrieval was performed.

    (From Figure 3 of Xiao et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Image courtesy of Rudolf Jung by Abreview.
    ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

    See Abreview

  • Western blot - Anti-Collagen III antibody (ab7778)
    Western blot - Anti-Collagen III antibody (ab7778)
    Anti-Collagen III antibody (ab7778) at 1/1000 dilution + Human collagen III at 0.1 µg

    Predicted band size: 138 kDa



    Western Blot produced under denaturing and reducing conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

    ab7778 at 1:400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 (ab64214) at 99-100°C - 20 minutes for antigen retrieval.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry of ab7778. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

    Ab53088 staining Human skin (ab30166). Staining is localised to the extracellular matrix.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Protocols

  • ELISA to quantify collagen

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (331)

Publishing research using ab7778? Please let us know so that we can cite the reference in this datasheet.

ab7778 has been referenced in 331 publications.

  • Qin T  et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). PubMed: 31386888
  • Dou F  et al. Chrysophanol ameliorates renal interstitial fibrosis by inhibiting the TGF-ß/Smad signaling pathway. Biochem Pharmacol N/A:114079 (2020). PubMed: 32511988
  • Danalache M  et al. Biochemical changes of the pericellular matrix and spatial chondrocyte organization-Two highly interconnected hallmarks of osteoarthritis. J Orthop Res N/A:N/A (2020). PubMed: 32301522
  • Xie X  et al. Trib1 Contributes to Recovery From Ischemia/Reperfusion-Induced Acute Kidney Injury by Regulating the Polarization of Renal Macrophages. Front Immunol 11:473 (2020). PubMed: 32265926
  • Cheng L  et al. Hydration-Enhanced Lubricating Electrospun Nanofibrous Membranes Prevent Tissue Adhesion. Research (Wash D C) 2020:4907185 (2020). PubMed: 32270140
View all Publications for this product

Customer reviews and Q&As

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1-10 of 43 Abreviews or Q&A

Question

I have several questions about your product 'ab7778'.

Although I used ab7778, I didn't get a result about non-reducing and native form of collagen 3.

Please let me know the way you confirmed native collagen 3.

I’d appreciate if you give me a detailed protocol containing information about gel, what I should run how much volt and how many hours,

protein amount, how to transfer and blocking, antibodies incubation, visualization and so on...

I hope to give a answer as soon as possible.

Yours sincerely,

Read More

Abcam community

Verified customer

Asked on Jan 21 2013

Answer

Thank you for contacting us.

I can confirm that any general western blotting protocol would work with this antibody. Please check the following conditions;

- the conditions should be native and non-reduced.
- we normally recommend loading 30-50ug of protein
- Do not boil the samples, heat for 10-15 mins at 70-80C.
- Try 8% gel
- Antibody should be incubated overnight at 4C.

The following publication might also help;

http://www.ncbi.nlm.nih.gov/pubmed/22011392?dopt=Abstract

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 21 2013

Immunohistochemistry (Frozen sections) abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Aug 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (lymph node)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: pepsin
Permeabilization
Yes - 0.05% Tween 20
Specification
lymph node
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Le Zhen

Verified customer

Submitted Sep 17 2019

Western blot abreview for Anti-Collagen III antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblast)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
10 µg
Specification
Fibroblast
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 21 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ankle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
Permeabilization
No
Specification
Ankle
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 28 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
Permeabilization
No
Specification
Skin
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 29 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
10% Formalin
Read More

Abcam user community

Verified customer

Submitted Nov 13 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Equus Cell (Tenocyte)
Permeabilization
No
Specification
Tenocyte
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Permeabilization
No
Specification
Dermal Fibroblast
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Paraformaldehyde
Read More

Adrian Djalali Cuevas

Verified customer

Submitted Sep 22 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Urethera)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Urethera
Blocking step
Rodent Block Rat (Biocare Medical) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Read More

Premier Laboratory

Verified customer

Submitted May 18 2016

1-10 of 43 Abreviews or Q&A

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