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  1. Link

    collagen-iii-antibody-ab7778.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen III antibody (ab7778)

  • Datasheet
  • SDS
Reviews (24)Q&A (25)References (448)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Western blot - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

Key features and details

  • Rabbit polyclonal to Collagen III
  • Suitable for: ICC/IF, IP, IHC-Fr, ELISA, IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Collagen III antibody [EPR17673] (ab184993)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Collagen III antibody
    See all Collagen III primary antibodies
  • Description

    Rabbit polyclonal to Collagen III
  • Host species

    Rabbit
  • Specificity

    This type specific collagen antibody only recognizes 3D epitopes. Negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-Fr, ELISA, IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Collagen III aa 1-1466.
    Database link: P02461

  • Positive control

    • Natural Cow Collagen III protein (ab7528) can be used as a positive control in WB. Human skin. Human testicle tissue.
  • General notes

    For more protocol tips, please see: https://www.abcam.com/protocols/collagen

    At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.8766% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Native Cow Collagen III protein (ab7528)
    • Native Human Collagen III protein (ab7535)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • 10x Citrate Buffer pH 6.0 (ab64214)
    • Anti-MMP2 antibody [6E3F8] (ab86607)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
Use at an assay dependent concentration. PubMed: 18385800
IP
Use at an assay dependent concentration.
IHC-Fr (5)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

This product was assayed against 1.0 ug of Collagen III in a direct ELISA using Peroxidase conjugated Goat anti-Rabbit and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. This product can also be used with Biotin Conjugated Anti-Collagen III (ab6580) in a sandwich ELISA.

IHC-P (13)
1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration. PubMed: 19036760
WB (3)
1/5000 - 1/10000. Predicted molecular weight: 138 kDa.
IF
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration. PubMed: 18385800
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

This product was assayed against 1.0 ug of Collagen III in a direct ELISA using Peroxidase conjugated Goat anti-Rabbit and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. This product can also be used with Biotin Conjugated Anti-Collagen III (ab6580) in a sandwich ELISA.

IHC-P
1/50 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration. PubMed: 19036760
WB
1/5000 - 1/10000. Predicted molecular weight: 138 kDa.
IF
Use at an assay dependent concentration.

Target

  • Function

    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in disease

    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02461 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 510833 Cow
    • Entrez Gene: 1281 Human
    • Entrez Gene: 12825 Mouse
    • Entrez Gene: 84032 Rat
    • Omim: 120180 Human
    • SwissProt: P04258 Cow
    • SwissProt: P02461 Human
    • SwissProt: P08121 Mouse
    • SwissProt: P13941 Rat
    • Unigene: 443625 Human
    • Unigene: 249555 Mouse
    • Unigene: 3247 Rat
    see all
  • Alternative names

    • Alpha 1 type III collagen antibody
    • Alpha1 (III) collagen antibody
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha 1 chain antibody
    • Collagen type III alpha antibody
    • Collagen, fetal antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Xiao, H. et al PLoS One. 2013 Apr 9;8(4):e60173. doi: 10.1371/journal.pone.0060173. Print 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    A2AR activity affected UUO (unilateral ureteral obstruction)-induced deposition of collagen III

    (A) Representative immunohistochemistry of Collagen III) (Col III) from the A2AR KO and WT mice, at day 3, 7 and 14 post-UUO or Sham, following treatment of CGS21680 (CGS) or vehicle (Veh). Scale bar  = 50 µm, 400×. 

    Collagen III in rat kidney tissue is detected ab7778 at 1/400 dilution. Pressure cooker mediated antigen retrieval was performed.

    (From Figure 3 of Xiao et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Image courtesy of Rudolf Jung by Abreview.
    ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

    See Abreview

  • Western blot - Anti-Collagen III antibody (ab7778)
    Western blot - Anti-Collagen III antibody (ab7778)
    Anti-Collagen III antibody (ab7778) at 1/1000 dilution + Human collagen III at 0.1 µg

    Predicted band size: 138 kDa



    Western Blot produced under denaturing and reducing conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

    ab7778 at 1:400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 (ab64214) at 99-100°C - 20 minutes for antigen retrieval.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry of ab7778. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

    Ab53088 staining Human skin (ab30166). Staining is localised to the extracellular matrix.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Protocols

  • ELISA to quantify collagen

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (448)

Publishing research using ab7778? Please let us know so that we can cite the reference in this datasheet.

ab7778 has been referenced in 448 publications.

  • Bochon K  et al. The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells. Int J Stem Cells 14:33-46 (2021). PubMed: 33122467
  • He J  et al. Mechanical stretch promotes hypertrophic scar formation through mechanically activated cation channel Piezo1. Cell Death Dis 12:226 (2021). PubMed: 33649312
  • Sántha P  et al. Morphological Heterogeneity in Pancreatic Cancer Reflects Structural and Functional Divergence. Cancers (Basel) 13:N/A (2021). PubMed: 33672734
  • Li Z  et al. MicroRNA-497-5p downregulation inhibits cell viability, reduces extracellular matrix deposition and induces apoptosis in human hyperplastic scar fibroblasts by regulating Smad7. Exp Ther Med 21:384 (2021). PubMed: 33680106
  • Fang L  et al. Naoxintong accelerates diabetic wound healing by attenuating inflammatory response. Pharm Biol 59:252-261 (2021). PubMed: 33684026
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Equus Cell (Tenocyte)
Permeabilization
No
Specification
Tenocyte
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen III antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Permeabilization
No
Specification
Dermal Fibroblast
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Adrian Djalali Cuevas

Verified customer

Submitted Sep 22 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen III antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Mouse Cell (Hepatocyte)
Specification
Hepatocyte
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2014

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