Overview

  • Product name
    Anti-Collagen III antibody [FH-7A]
    See all Collagen III primary antibodies
  • Description
    Mouse monoclonal [FH-7A] to Collagen III
  • Host species
    Mouse
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-Fr, WB, IHC-P, ICC/IF, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • IHC: Human skin sections. WB: recombinant collagen III.
  • General notes

    Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

     

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab6310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25136258
Indirect ELISA Use at an assay dependent concentration.

Target

  • Function
    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in disease
    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type III collagen antibody
    • Alpha1 (III) collagen antibody
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha 1 chain antibody
    • Collagen type III alpha antibody
    • Collagen, fetal antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Images

  • All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

    Lane 1 : Reduced, denaturated Collagen III protein
    Lane 2 : Native Collagen III protein

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Goat anti-mouse HRP at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 1 minute


    This image was generated using the ascites version of the product.

  • Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

  • ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

    This image was generated using the ascites version of the product.

    See Abreview

  • Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:
  • Ho TC  et al. PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells. Stem Cell Res Ther 10:2 (2019). Read more (PubMed: 30606221) »
  • Liu B  et al. Costunolide inhibits pulmonary fibrosis via regulating NF-kB and TGF-ß1/Smad2/Nrf2-NOX4 signaling pathways. Biochem Biophys Res Commun 510:329-333 (2019). Read more (PubMed: 30709583) »
See all 82 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-10 of 10 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (femoral vein)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
Yes - Triton X
Specification
femoral vein
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 23 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
skin tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 24 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 24 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 24 2018

Application
ELISA
Sample
Human Purified protein (purified protein and cell culture supernatant)
Specification
purified protein and cell culture supernatant
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Sandwich (Capture)

Patricia Carulla

Verified customer

Submitted Jun 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester human decidua)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0.1% (W/V) Trypsin
Permeabilization
No
Specification
First trimester human decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Ruhul Choudhury

Verified customer

Submitted Feb 13 2017

Application
Western blot
Sample
Human Purified protein (ab7535)
Loading amount
5 µg
Specification
ab7535
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Michaela Leyh

Verified customer

Submitted Feb 04 2011

Application
ELISA
Sample
Human Purified protein (Pepsin-extracted Human placental collagen III)
Specification
Pepsin-extracted Human placental collagen III
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Direct

Ben Maddox

Verified customer

Submitted Dec 14 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 31 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (preovulatory follicle and whole ovary)
Specification
preovulatory follicle and whole ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: From Vecta

Ms. Birgitta Weijdegard

Verified customer

Submitted Oct 10 2006

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