Anti-Collagen IV alpha 1 antibody (ab189408)

Overview

  • Product name

    Anti-Collagen IV alpha 1 antibody
    See all Collagen IV alpha 1 primary antibodies
  • Description

    Rabbit polyclonal to Collagen IV alpha 1
  • Host species

    Rabbit
  • Specificity

    ab189408 recognizes extracellular - basement membranes.
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen IV alpha 1. Isolated from Human placenta
    Database link: P02462

  • Positive control

    • Human kidney tissue, glomerulus.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab189408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75 - 1/100.

Sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.

WB 1/100 - 1/1000. Predicted molecular weight: 161 kDa.
IHC-Fr 1/25 - 1/100.

Fix in methanol, aceton or 1% paraformaldehyde. A PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended.

ICC/IF Use at an assay dependent concentration.

Methanol fixation recommended.

Target

  • Relevance

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Database links

  • Alternative names

    • Arresten antibody
    • COL4A1 NC1 domain antibody
    • Collagen alpha-1(IV) chain antibody
    • Collagen IV alpha-1 polypeptide antibody
    • Collagen of basement membrane, alpha 1 chain antibody
    • HANAC antibody
    • POREN1 antibody
    see all

Images

  • Immunohistochemistry of formalin-fixed, paraffin-embedded Human kidney, glomerulus, labeling Collagen IV alpha 1 with ab189408 at a 1/75 dilution.

References

ab189408 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189408.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up