Anti-Collagen IV alpha 1 antibody - N-terminal (ab135802)

Overview

  • Product name

    Anti-Collagen IV alpha 1 antibody - N-terminal
    See all Collagen IV alpha 1 primary antibodies
  • Description

    Rabbit polyclonal to Collagen IV alpha 1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Collagen IV alpha 1 aa 27-54 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

    • Hela cell line and Mouse lung tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135802 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab135802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 161 kDa.

Target

  • Relevance

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Database links

  • Alternative names

    • Arresten antibody
    • COL4A1 NC1 domain antibody
    • Collagen alpha-1(IV) chain antibody
    • Collagen IV alpha-1 polypeptide antibody
    • Collagen of basement membrane, alpha 1 chain antibody
    • HANAC antibody
    • POREN1 antibody
    see all

Images

  • Anti-Collagen IV alpha 1 antibody - N-terminal (ab135802) at 1/50 dilution + Hela cell line lysates at 35 µg

    Predicted band size: 161 kDa

  • Anti-Collagen IV alpha 1 antibody - N-terminal (ab135802) at 1/50 dilution + Mouse lung tissue lysates at 35 µg

    Predicted band size: 161 kDa

References

This product has been referenced in:

  • Ma Y  et al. Comparison of diabetic nephropathy between male and female eNOS-/-db/db mice. Am J Physiol Renal Physiol 316:F889-F897 (2019). Read more (PubMed: 30810354) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (femoral artery)
Permeabilization
Yes - 0,1% Triton-X
Specification
femoral artery
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Miss. Julia Werner

Verified customer

Submitted Nov 24 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (1=aorta, 2=heart, 3=lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
15 µg
Specification
1=aorta, 2=heart, 3=lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up