Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)
Key features and details
- Rabbit polyclonal to Collagen IV alpha 1 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Collagen IV alpha 1 antibody - N-terminal
See all Collagen IV alpha 1 primary antibodies -
Description
Rabbit polyclonal to Collagen IV alpha 1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Collagen IV alpha 1 (N terminal). The exact sequence is proprietary.
Database link: P02462 -
Positive control
- IHC-P: Mouse brain tissue. WB: SK-N-SH whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 161 kDa. |
Target
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Relevance
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. -
Database links
- Entrez Gene: 1282 Human
- Entrez Gene: 12826 Mouse
- Omim: 120130 Human
- SwissProt: P02462 Human
- SwissProt: P02463 Mouse
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Alternative names
- Arresten antibody
- COL4A1 NC1 domain antibody
- Collagen alpha-1(IV) chain antibody
see all
Images
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Western blot - Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616) at 1/500 dilution + SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 161 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)Paraffin-embedded mouse brain tissue stained for Collagen IV alpha 1 with ab227616 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227616 has not yet been referenced specifically in any publications.
