Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Collagen IV alpha 1 antibody - N-terminal
See all Collagen IV alpha 1 primary antibodies -
Description
Rabbit polyclonal to Collagen IV alpha 1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Collagen IV alpha 1 (N terminal). The exact sequence is proprietary.
Database link: P02462 -
Positive control
- IHC-P: Mouse brain tissue. WB: SK-N-SH whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 161 kDa. |
Target
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Relevance
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. -
Database links
- Entrez Gene: 1282 Human
- Entrez Gene: 12826 Mouse
- Omim: 120130 Human
- SwissProt: P02462 Human
- SwissProt: P02463 Mouse
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Alternative names
- Arresten antibody
- COL4A1 NC1 domain antibody
- Collagen alpha-1(IV) chain antibody
see all
Images
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Western blot - Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616) at 1/500 dilution + SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 161 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV alpha 1 antibody - N-terminal (ab227616)Paraffin-embedded mouse brain tissue stained for Collagen IV alpha 1 with ab227616 at 1/500 dilution in immunohistochemical analysis.
References
ab227616 has not yet been referenced specifically in any publications.
