Overview

  • Product name

    Anti-Collagen IV antibody [1043]
    See all Collagen IV primary antibodies
  • Description

    Mouse monoclonal [1043] to Collagen IV
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat, Rabbit, Cow, Pig
  • Immunogen

    Full length protein corresponding to Human Collagen IV.

  • General notes

    In IHC, recognizes the extracellular – basement membranes.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27th October 2017. The following lots are still unpurified and still in stock as of 27th October 2017- GR275641-7, GR275641-13, and GR275641-14. Lot numbers other than GR275641-7, GR275641-13, and GR275641-14 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab86042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Use under non reducing condition. Predicted molecular weight: 160 kDa.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC 1/50 - 1/100. Methanol fixed.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Tissue specificity

    Highly expressed in placenta.
  • Involvement in disease

    Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • Sequence similarities

    Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • Domain

    Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • Post-translational
    modifications

    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arresten antibody
    • BSVD antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • COL4A1 NC1 domain antibody
    • COL4A2 antibody
    • COL4A3 antibody
    • COL4A4 antibody
    • COL4A5 antibody
    • collagen alpha-1(IV) chain antibody
    • Collagen IV Alpha 1 Polypeptide antibody
    • Collagen IV Alpha 2 Polypeptide antibody
    • Collagen Of Basement Membrane Alpha 1 Chain antibody
    • Collagen Of Basement Membrane Alpha 2 Chain antibody
    • Collagen Type IV Alpha 1 antibody
    • collagen type IV alpha 1 chain antibody
    • Collagen Type IV Alpha 2 antibody
    • Collagen Type IV Alpha 3 antibody
    • Collagen Type IV Alpha 4 antibody
    • Collagen Type IV Alpha 5 antibody
    • RATOR antibody
    see all

Images

  • ab86042 (TCS version) staining Collagen IV in Human kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • Immunohistochemistry on frozen section of Human colon with ab86042 (TCS version).

  • Immunohistochemistry on paraffin section of Human skin

References

This product has been referenced in:

  • Henshaw FR  et al. Topically applied connective tissue growth factor/CCN2 improves diabetic preclinical cutaneous wound healing: potential role for CTGF in human diabetic foot ulcer healing. J Diabetes Res 2015:236238 (2015). Read more (PubMed: 25789327) »
  • Havenith MG  et al. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87:123-8 (1987). Read more (PubMed: 3305430) »
See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Your interpretation of the information is correct. It has been found with this antibody that it can be used to detect the denatured protein but not with a reducing agent present. This is illustrated in the following reference:

Havenith MG et al. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87:123-8 (1987). PubMed: 3305430

This reference describes the production of the 1043 clone and has an image of a western blot (figure 3, lanes 8 and 9). The Materials and Methods describes purification of collagen IV and refers to Laemmli 1970 for preparation of the samples for SDS-PAGE. I am assuming the samples were heat-denatured, though this is not stated in the article. A signal in Western blotting was only obtained with the antibody from the sample that was not reduced with beta-mercaptoethanol, lane 9 of figure 3.

Read More

Answer

Thank you for contacting us.

This antibody has not been tested in rat. A BLAST search of the immunogen showed 84% homology with the rat sequence, so it is likely to cross-react.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Ideally you would need a mouse anti-rabbit collagen IV antibody. If this is not possible, then I would try looking for a mouse anti-human collagen IV that has been proven to react with rabbit collagen IV since unfortunately, we do not have this in our catalogue.

Read More

Answer

I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?

With regards to finding an antibody which is likely to work rabbit, I have managed to find the protein sequences of the alpha chain 1,2 and 4 of rabbit collagen IV at:

http://www.uniprot.org/uniprot/Q7M2X2
http://www.uniprot.org/uniprot/Q7M2X5
http://www.uniprot.org/uniprot/P55787

respectively.

However, some of these sequences are incomplete. For the amino acid data we do have, it appears that there is a relatively high % homology between rabbit and human however because, our data is incomplete and it appears that the rabbit protein may be smaller, I am unable to predict whether an antibody is likely to work or not in rabbit.

If you would like to test one of the antibodies from our catalogue we have 7 mouse monoclonals which are guaranteed to work in IHC to choose from. If you would like to test one of them, please contact us letting us know which one you would like to test.

Read More

Answer

As previously discussed with you, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. It is regrettably very difficult to suggest antibodies to customers that we can guarantee to work in one species and definitely not in another. Some antibodies can even show inconsistent results in one particular species, so could work for one customer and not another.

Therefore, I can offer a discount off a future purchase if you buyab6311 now totest in mouse and submit feedback to us in the form of an Abreview. This is on the understanding that we cannot guarantee this antibody willnot detect in mouse.

For us, it does not matter whether the Abreview is positive or negative, we would just really like to receive your feedback of the results in mouse. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please I would appreciate if you could take time to read through the terms and conditions and then follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab6311 in mouse. I will then send a discount code. This code must be issued before purchasing ab6311 so please wait for my reply before ordering.

2. Purchase ab6311 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!Whether ab6311 turns out to be suitable orunsuitable for detection of Collagen IV in mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions and I would be happy to help you further. I look forward to hearing from you with confirmation that you would like to proceed with the offer.

Read More

Answer

Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or any other application). Unfortunately, as all the antibodies which might be applicable have been raised against the full length protein it is not possible to tell how likely it is that it will cross react.

As we discussed, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. However, as it is a monoclonal antibody, if it does not cross react it is likely to show this specificity consistently from batch to batch and may therefore be most worthwhile trying.

If you would like to try the antibodyab6311 or the other mouse monolconal ab86042 in Western blotting we would be able to offer you a testing discount.

This offer means that if you test the antibodywith mousesamples inWestern blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

When performing the western blot, it would be very helpful if you could show both the positive and negative data (i.e. blotting with the human sample alongside the mouse sample to make sure the antibody is working effectively).

If you would like to participate in this scheme please do let me know as a discount code needs to be issued prior to purchasing the antibody. Please also let me know which of the two antibodies you would like to test (ab6311 or ab86042).

I look forward to receiving your reply.

Read More

Answer

\It is recommended that 0.1 mM CaCl2 and 0.1 mM MgCl2 should be included in the primary antibody incubation buffer. If the initial antibody incubation buffer did not contain 0.1 mM CaCl2 and 0.1 mM MgCl2, I think it is worthwhile to try the staining one more time with this buffer. Thanks for your patience with the troubleshooting process. I look forward to hearing how things go.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up