Question (36956) | Anti-Collagen IV antibody (ab19808)

Go to datasheet (ab19808)

Question

Ordered and aliquotted ab6586 but then saw that Abcam recommends using the antibody in non-denatured conditions.

Answer

Thanks for your call today and for your questions about our collagen IV antibodies.
As we discussed, I would still recommend trying ab6586 with your standard Western blotting conditions. We recommend non-denatured conditions because the affinity of the antibody is higher in these conditions, but I did find user-submitted Abreviews in which the antibody was used in denatured conditions and the results were satisfactory (rated "Excellent" by the reviewers)-
https://www.abcam.com/index.html?datasheet=6586&tab=abreviews&intabreviewid=7703
https://www.abcam.com/index.html?datasheet=6586&tab=abreviews&intabreviewid=8531
Please note that the expected band size in these conditions is between 200 and 250 kDa.
I hope this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help. I apologize for any confusion regarding the information on our datasheet, and I will see if we can update the Western blot application information.

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