Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Specification
brain
Fixative
Formaldehyde
Permeabilization
No
Blocking step
TNB as blocking agent for 30 minute(s) · Concentration: 0.25% · Temperature: 22°C

Other product details

Dilution
1/250
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TNB

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit bio then perkin Elmer TSA kit with Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500

Additional data

Additional Notes
This antibody works well once it is amplified using the Perkin Elmer TSA biotin kit. Primary is left on over night at 4 degrees. Tissue is lightly fixed in 4% PFA by perfusion (10-15ml) - not immersed in PFA. No AR needed. Ab stains basement membrane of blood vessels in the brain- using Alexa Fluor 546 (red). Green is GFP tumour growing around blood vessels.

Abcam user community

Verified customer

Submitted Feb 10 2010

Sign up