Non-Abcam antibody was used: anti rabbit bio then perkin Elmer TSA kit with Ale Host species: Goat Clonality: Polyclonal Conjugation: Biotin
This antibody works well once it is amplified using the Perkin Elmer TSA biotin kit. Primary is left on over night at 4 degrees. Tissue is lightly fixed in 4% PFA by perfusion (10-15ml) - not immersed in PFA. No AR needed. Ab stains basement membrane of blood vessels in the brain- using Alexa Fluor 546 (red). Green is GFP tumour growing around blood vessels.
Abcam user community
Submitted Feb 10 2010
Get resources and offers direct to your inboxSign up