Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse ear vessel)
Specification
Mouse ear vessel
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0,1%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1%

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200

Additional data

Additional Notes
whole mount immunofluorescence of mouse ear, 3D reconstruction of Z.stack

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Submitted Jun 04 2010

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