Western blot abreview for Anti-Collagen IV antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (pancreatic cancer tissue sample)
Loading amount
25 µg
Specification
pancreatic cancer tissue sample
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: Amersham ECL Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 160 kDa Non-specific: ca.250, 80 kDa

Additional data

Additional Notes
Collagen predicted size: 160kDa, other observed bands maybe due to post- translational collagen modifications.

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Submitted Jul 07 2010

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