Immunohistochemistry - Free Floating abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain blood vessels)
Specification
brain blood vessels

Other product details

Dilution
1/250
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% normal goat serum, 0.2% Triton X, PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Prior to staining I perform antigen retrieval. Sections are incubated at 95 degrees C for 40 minutes in a 10mM Sodium Citrate buffer with 0.05% Tween 20 and pH 6.0. They are rinsed 3x in PBS. Antigen retrieval makes the sections curl up so very gentle mounting is necessary.

Secondary antibody is incubated for 6h at RT.

Ms. Christina Whiteus

Verified customer

Submitted Oct 20 2010

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