48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% normal goat serum, 0.2% Triton X, PBS
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488 Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 488
Prior to staining I perform antigen retrieval. Sections are incubated at 95 degrees C for 40 minutes in a 10mM Sodium Citrate buffer with 0.05% Tween 20 and pH 6.0. They are rinsed 3x in PBS. Antigen retrieval makes the sections curl up so very gentle mounting is necessary.
Secondary antibody is incubated for 6h at RT.
Ms. Christina Whiteus
Submitted Oct 20 2010
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