Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse brain cells)
Specification
Mouse brain cells
Fixative
Alcool-Formol-Acetate (AFA)
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: PBS BSA 3%

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 donkey anti-rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Green: Collagen IV
Blue: Dapi

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Submitted Nov 15 2011

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