Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Alcool-Formol-Acetate (AFA)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: PBS BSA 3%

Secondary antibody

Name
Non-Abcam antibody was used: Dako EnVision+ System-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
left side: normal mouse brain
right side: human glioblastoma xenograft

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Submitted Nov 23 2011

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