Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary gland)
Specification
mammary gland
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
I obtained better results with methacarnoy fixative than PFA (less background).

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Submitted Jan 26 2006

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