Immunohistochemistry (Frozen sections) abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% Triton
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NGS 3% Triton 0.5% PBS -/- 1%
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor goat-anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
An antigen retrieval step (for 50ml: 5ml of Citrate buffer 10X ph6, 25´l of tween 100%, 45ml H2O, 35 minutes at 98¯C) was performed prior to primary antibody incubation.
PubMed ID
25331846: View

Ms. Diana Cifuentes

Verified customer

Submitted Dec 01 2014

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