Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Collagen IV antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (femoral ertery)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X
Specification
femoral ertery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rabbit IgG secondary antibody Alexa Fluo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

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Submitted Dec 16 2015

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