Key features and details
- Rabbit polyclonal to Collagen IV
- Suitable for: ELISA, RIA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Collagen IV antibody
See all Collagen IV primary antibodies
DescriptionRabbit polyclonal to Collagen IV
Tested applicationsSuitable for: ELISA, RIAmore details
Species reactivityReacts with: Human
Full length native protein (purified): Collagen type IV extracted and purified from human placenta
- IHC-P: human placenta FFPE tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab21295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
Tissue specificityHighly expressed in placenta.
Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
Sequence similaritiesBelongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Arresten antibody
- BSVD antibody
- CO4A1_HUMAN antibody
ab21295 has been referenced in 6 publications.
- Kim YC et al. Renoprotective effects of a novel cMet agonistic antibody on kidney fibrosis. Sci Rep 9:13495 (2019). PubMed: 31530851
- Song M et al. Preparation and characterization of acellular adipose tissue matrix using a combination of physical and chemical treatments. Mol Med Rep 17:138-146 (2018). PubMed: 29115567
- Ross JB et al. Identification of molecular determinants of primary and metastatic tumour re-initiation in breast cancer. Nat Cell Biol 17:651-64 (2015). IHC . PubMed: 25866923
- Jean J et al. Effects of serum-free culture at the air-liquid interface in a human tissue-engineered skin substitute. Tissue Eng Part A 17:877-88 (2011). IHC-Fr ; Human . PubMed: 21067466
- Rouabhia M & Allaire P Gingival mucosa regeneration in athymic mice using in vitro engineered human oral mucosa. Biomaterials 31:5798-804 (2010). IHC-Fr ; Human . PubMed: 20434214
- Yin Z et al. Analysis of pairwise cell interactions using an integrated dielectrophoretic-microfluidic system. Mol Syst Biol 4:232 (2008). ChIP . PubMed: 19096359