Anti-Collagen IV antibody [COL-94] (ab6311)

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab6311 in mouse.

DISCOUNT CODE: 100%ABR-E76ZQ
Expiration date:13/11/11

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your reply and further enquiry which has been forwarded to me as my colleague Karin is currently away from the office.

As Karin previously discussed with you, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. It is regrettably very difficult to suggest antibodies to customers that we can guarantee to work in one species and definitely not in another. Some antibodies can even show inconsistent results in one particular species, so could work for one customer and not another.

Therefore, I can offer a discount off a future purchase if you buyab6311 now totest in mouse and submit feedback to us in the form of an Abreview. This is on the understanding that we cannot guarantee this antibody willnot detect in mouse.

For us, it does not matter whether the Abreview is positive or negative, we would just really like to receive your feedback of the results in mouse. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please I would appreciate if you could take time to read through the terms and conditions and then follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab6311 in mouse. I will then send a discount code. This code must be issued before purchasing ab6311 so please wait for my reply before ordering.

2. Purchase ab6311 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!Whether ab6311 turns out to be suitable orunsuitable for detection of Collagen IV in mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions and I would be happy to help you further. I look forward to hearing from you with confirmation that you would like to proceed with the offer.

Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or any other application). Unfortunately, as all the antibodies which might be applicable have been raised against the full length protein it is not possible to tell how likely it is that it will cross react.

As we discussed, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. However, as it is a monoclonal antibody, if it does not cross react it is likely to show this specificity consistently from batch to batch and may therefore be most worthwhile trying.

If you would like to try the antibodyab6311 or the other mouse monolconal ab86042 in Western blotting we would be able to offer you a testing discount.

This offer means that if you test the antibodywith mousesamples inWestern blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

When performing the western blot, it would be very helpful if you could show both the positive and negative data (i.e. blotting with the human sample alongside the mouse sample to make sure the antibody is working effectively).

If you would like to participate in this scheme please do let me know as a discount code needs to be issued prior to purchasing the antibody. Please also let me know which of the two antibodies you would like to test (ab6311 or ab86042).

I look forward to receiving your reply.

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with ab6311. As you have had success with ab6586, I would be happy to replace the product. Please could you confirm the lot number and date of order so I can issue the replacement.

Thank you in advance.

Thank you for your enquiry regarding ab6311 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.

1) Could you please specify the species of the samples and the tissue type?

2) The batch number your provided is invalid: 3002101000 Please check your record again and confirm the valid batch?

3) Could you please confirm if you purchased this product directly from Abcam or via a distributor? It would be very useful for me to know the Abcam Order Number and the date of the purchase.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

Vielen Dank für Ihren Anruf. Es tut mir leid, dass Sie wieder Probleme mit dem Collagen-Antikörper ab6311 hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung mit ab86042 für Sie in Auftrag gegeben. Sie hat die Referenznummer xxx und sollte in den nächsten Tagen bei Ihnen ankommen. Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Thank you for your enquiry. I am sorry for the confusion. My colleague was referring to a publication where ab6311 has been applied under denaturing, reduced conditions. However, this antibody was raised to specifically recognizes the native, undenatured form of human collagen type IV. I would therefore recommend that the antibody is applied in this manner. The Collagen group of proteins have many side chains and their migration under denaturing gels is not very predictable. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. None of our Collagen IV antibodies have been tested together in a sandwich ELISA. They were tested in indirect ELISAs. We ship to Brazil using FedEx International. Please place your order online, or by email, phone or fax (details below). Abcam Ltd 332 Cambridge Science Park Milton Road Cambridge CB4 0FW UK Email:orders@abcam.com Phone:+44 1223 472030 Fax:+44 1223 472038 Office hours: 08.30 - 17.00 hrs (UK) Monday to Friday