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Anti-Collagen IV antibody [COL-94] (ab6311)

Reviews (10)Q&A (14)References (36)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)

Key features and details

  • Mouse monoclonal [COL-94] to Collagen IV
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Collagen IV antibody [COL-94]
    See all Collagen IV primary antibodies

  • Description

    Mouse monoclonal [COL-94] to Collagen IV

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primatesDoes not react with: Rat, Sheep, Goat, Chicken, Cat

  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen IV.
    Database link: P02462

  • Epitope

    Recognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.

  • Positive control

    • Natural Human Collagen IV protein (ab7536) can be used as a positive control in WB. Human skin tissue.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3230927 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (5)
Use a concentration of 4 - 8 µg/ml.

The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Notes
IHC-P
Use a concentration of 4 - 8 µg/ml.

The antibody was developed using biotin/ExtrAvidin®-Peroxidase.

Target

  • Function

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

  • Tissue specificity

    Highly expressed in placenta.

  • Involvement in disease

    Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.

  • Sequence similarities

    Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

  • Domain

    Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

  • Post-translational
    modifications

    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Arresten antibody
    • BSVD antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • COL4A1 NC1 domain antibody
    • COL4A2 antibody
    • COL4A3 antibody
    • COL4A4 antibody
    • COL4A5 antibody
    • collagen alpha-1(IV) chain antibody
    • Collagen IV Alpha 1 Polypeptide antibody
    • Collagen IV Alpha 2 Polypeptide antibody
    • Collagen Of Basement Membrane Alpha 1 Chain antibody
    • Collagen Of Basement Membrane Alpha 2 Chain antibody
    • Collagen Type IV Alpha 1 antibody
    • collagen type IV alpha 1 chain antibody
    • Collagen Type IV Alpha 2 antibody
    • Collagen Type IV Alpha 3 antibody
    • Collagen Type IV Alpha 4 antibody
    • Collagen Type IV Alpha 5 antibody
    • RATOR antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)

    Immunohistochemical analysis of paraffin-embedded human tongue tissue staining Collagen IV with ab6311 at 8µg/ml. The antibody was developed using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)This image is courtesy of an abreview submitted by Birgitta Weijdegard, Sahlgrenska Cancer Center.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with unpurified ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
    Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.

    This image was generated using the ascites version of the product.

    See Abreview

References (36)

Publishing research using ab6311? Please let us know so that we can cite the reference in this datasheet.

ab6311 has been referenced in 36 publications.

  • Campbell-Thompson M & Tang SC Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes. Front Endocrinol (Lausanne) 12:644826 (2021). PubMed: 33981285
  • Kostov K & Blazhev A Serum Anti-Collagen IV IgM and IgG Antibodies as Indicators of Low Vascular Turnover of Collagen IV in Patients with Long-Term Complications of Type 2 Diabetes. Diagnostics (Basel) 11:N/A (2021). PubMed: 34069322
  • Ashworth JC  et al. Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro. Matrix Biol 85-86:15-33 (2020). PubMed: 31295578
  • Yemanyi F  et al. Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 61:16 (2020). PubMed: 33170205
  • Yemanyi F  et al. Crosslinked Extracellular Matrix Stiffens Human Trabecular Meshwork Cells Via Dysregulating ß-catenin and YAP/TAZ Signaling Pathways. Invest Ophthalmol Vis Sci 61:41 (2020). PubMed: 32832971
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

1-10 of 14 Q&A

Question

Thanks for your reply. BTW, I am interested in performing IHC on rabbit tissue. That is what we are talking about, correct?
I will check the biocompare website and get back to you if we can not find what we are looking for.
Sounds like a mouse anti-human Ab would be a reasonable guess, right?

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Answer

Thank you for contacting us.

Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Ideally you would need a mouse anti-rabbit collagen IV antibody. If this is not possible, then I would try looking for a mouse anti-human collagen IV that has been proven to react with rabbit collagen IV since unfortunately, we do not have this in our catalogue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Thank you for your swift reply.
We are interested in your offer and would like to participate if you can find an antibody that might work. we do realize that the restrictions of working with rabbit tissue and paraffinized sections does not make the process easier. The use of frozen tissue sections would be a possibility if that is likely to be the only option that works, but it would not be our preferred choice.
BTW, the Acris antibody was purchased through Labforce.

I unfortunately do not have the protein sequences for rabbit collagen IV. Can try to look those up if any info exists, i am just not sure when I will be able to get to that.
Thanks again for the reply.

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Answer

Thank you for contacting us.

I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?

With regards to finding an antibody which is likely to work rabbit, I have managed to find the protein sequences of the alpha chain 1,2 and 4 of rabbit collagen IV at:

http://www.uniprot.org/uniprot/Q7M2X2
http://www.uniprot.org/uniprot/Q7M2X5
http://www.uniprot.org/uniprot/P55787

respectively.

However, some of these sequences are incomplete. For the amino acid data we do have, it appears that there is a relatively high % homology between rabbit and human however because, our data is incomplete and it appears that the rabbit protein may be smaller, I am unable to predict whether an antibody is likely to work or not in rabbit.

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers to see if they have an anti-collagen IV predicted to work in rabbit. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

Otherwise, if you would like to test one of the antibodies from our catalogue we have 7 mouse monoclonals which are guaranteed to work in IHC to choose from. If you would like to test one of them, please contact us letting us know which one you would like to test.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

we would like to buy the anti body AB6311 under the collaboration discount programm. I am therefore waiting for the discount number.

Best Regards,

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Answer

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab6311 in mouse.

DISCOUNT CODE: 100%ABR-E76ZQ
Expiration date:13/11/11

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

we will be interested by buying AB6311. But I must specify that we want to use the antibody for Immunofluorescence. We are cultivating human cells that are producing collagen IV in a mouse collagen IV matrice. We want to distinguish the human collagen to the mouse one. We expect the human one to be localize around the cells, where the mouse one will recover the whole substrate.

If this will meet the requirement for a test, we will be glad to participate to the collaboration program with you.

Aurelien Forget



Ph-D student

Universitaet Albert-Ludwig

BIOSS

Institut fur Makromolekular Chemie

Stefan-Meier-Str.31

79104 Freiburg

GERMANY

Tel: 00 49 761 203 6310

email: mailto:aurelien.forget@bioss.uni-freiburg.de





On Jul 6, 2012, at 2:30 PM, mailto:technical@abcam.com wrote:















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header








Dear Aurelien



Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or any other application). Unfortunately, as all the antibodies which might be applicable have been raised against the full length protein it is not possible to tell how likely it is that it will cross react.

As we discussed, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. However, as it is a monoclonal antibody, if it does not cross react it is likely to show this specificity consistently from batch to batch and may therefore be most worthwhile trying.

If you would like to try the antibodyab6311 or the other mouse monolconal ab86042 in Western blotting we would be able to offer you a testing discount.

This offer means that if you test the antibodywith mousesamples inWestern blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

When performing the western blot, it would be very helpful if you could show both the positive and negative data (i.e. blotting with the human sample alongside the mouse sample to make sure the antibody is working effectively).

If you would like to participate in this scheme please do let me know as a discount code needs to be issued prior to purchasing the antibody. Please also let me know which of the two antibodies you would like to test (ab6311 or ab86042).

I look forward to receiving your reply.

Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3928259


Best regards,
Karin

Karin Nordgren
Scientific Support Specialist
Abcam plc
https://www.abcam.com


Your original inquiry to Abcam:


Phone call requesting information on antibodies against Collagen IV able to differentiate between mouse and human target.








Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3928259]









Discover moreathttp://abcam.com

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Answer

Thank you for your reply and further enquiry which has been forwarded to me as my colleague Karin is currently away from the office.

As Karin previously discussed with you, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. It is regrettably very difficult to suggest antibodies to customers that we can guarantee to work in one species and definitely not in another. Some antibodies can even show inconsistent results in one particular species, so could work for one customer and not another.

Therefore, I can offer a discount off a future purchase if you buyab6311 now totest in mouse and submit feedback to us in the form of an Abreview. This is on the understanding that we cannot guarantee this antibody willnot detect in mouse.

For us, it does not matter whether the Abreview is positive or negative, we would just really like to receive your feedback of the results in mouse. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please I would appreciate if you could take time to read through the terms and conditions and then follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab6311 in mouse. I will then send a discount code. This code must be issued before purchasing ab6311 so please wait for my reply before ordering.

2. Purchase ab6311 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!Whether ab6311 turns out to be suitable orunsuitable for detection of Collagen IV in mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions and I would be happy to help you further. I look forward to hearing from you with confirmation that you would like to proceed with the offer.

Read More

Question

Phone call requesting information on antibodies against Collagen IV able to differentiate between mouse and human target.

Read More
Answer

Thank you for contacting us in regards to anti-Collagen IV antibodies.

As discussed over the phone, we do not currently have any antibodies which have been shown to detect human Collagen IV but not the mouse protein in Western blotting (or any other application). Unfortunately, as all the antibodies which might be applicable have been raised against the full length protein it is not possible to tell how likely it is that it will cross react.

As we discussed, ab6311, has been shown to not cross react with a number of different species but this does not necessarily mean that it will not cross react with mouse. However, as it is a monoclonal antibody, if it does not cross react it is likely to show this specificity consistently from batch to batch and may therefore be most worthwhile trying.

If you would like to try the antibodyab6311 or the other mouse monolconal ab86042 in Western blotting we would be able to offer you a testing discount.

This offer means that if you test the antibodywith mousesamples inWestern blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

When performing the western blot, it would be very helpful if you could show both the positive and negative data (i.e. blotting with the human sample alongside the mouse sample to make sure the antibody is working effectively).

If you would like to participate in this scheme please do let me know as a discount code needs to be issued prior to purchasing the antibody. Please also let me know which of the two antibodies you would like to test (ab6311 or ab86042).

I look forward to receiving your reply.

Read More

Question

This is regarding antibody Anti- Collagen IV(COL-94) (ab6311).My tissue of interest is human cornea.

I have purchased this antibody recently for IHC-P purpose and used it on 1:25,1:50, 1:100 dilution apart from 1:300 dilution as written in your TDS. Then I had used the collagen 4 antibody from some other lab and it worked fine ,the dilution that I used was 1:50.and the cat no. was ab6586 and it was specific.I have use the IHC treatment according to your protocol and used the same IHC kit(ab 80436) .I am sending you the results that I have got.

Kindly replace my antibody soon with ab6586, as I am stucked with my experiments.

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Answer

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with ab6311. As you have had success with ab6586, I would be happy to replace the product. Please could you confirm the lot number and date of order so I can issue the replacement.

Thank you in advance.

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Question

Dear Technical Team,

Hope this e-mail finds you well! Please find below a complaint from one of our customerto whom we suppliedthis antibody. The technical formis alsoattachedwith this mail. Request you to kindly look into it & adviseus accordingly todo further needful on it. Looking forward towards your valued reply/ comments on it. Thankingyou in advance.



With Best Regards,








Order Details









Antibody code *ab 6311







Lot number 3002101000





Purchase order number
or preferably Abcam order number 12-112/N-1286/Path/AKD/11-12/RS-Dt:-30-12-2011











General Information





Antibody storage conditions (temperature/reconstitution etc) -20







Description of the problem (high background, low signal, non-specific satining etc.) no positive signal







Sample (Species/Tissue/Cell Type/Cell Line etc.): Tissue













Fixation of sample (Ethanol/Methanol/Acetone/Para formaldehyde/Other/Duration etc.): 10% Formalin





Antigen retrieval (Enzymatic method, Heat mediated technique etc.): Heat mediated technique







Permeabilization step: Not applicable









Blocking conditions (Buffer/time period, Blocking agent etc.): Hydrogen peroxide half and hour then protein block 20 minutes(used IHC Kit ab80436)







Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step):collagen 4,manufacturer/mouse/1:25,1:50,1:100,1:200,incubation time was overnight at 4 degree,4-5 times washing in TBS







Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): used secondary antibody from IHC Kit ab80436/manufacturer/ EXPOSE Mouse and Rabbit Specific HRP/prediluted/half and hour at room temperature, 4-5 times washing in TBS.











Detection method: DAB



















Positive and negative controls used (please specify):positive control : skin tissue negative control: only secondary antibody







Optimization attempts (problem solving)









How many times have you tried the IHC?







4











Have you run a "No Primary" control?



√Yes No

















Do you obtain the same results every time?



√Yes No









What steps have you altered? I have changed the dilution of primary antibody from 1:200 to 1:25

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Answer

Thank you for your enquiry regarding ab6311 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.

1) Could you please specify the species of the samples and the tissue type?

2) The batch number your provided is invalid: 3002101000 Please check your record again and confirm the valid batch?

3) Could you please confirm if you purchased this product directly from Abcam or via a distributor? It would be very useful for me to know the Abcam Order Number and the date of the purchase.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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Question

ab6311, lot# GR42738-5 funktioniert leider auch nicht (IHC-Fr, human)

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Answer

Vielen Dank für Ihren Anruf. Es tut mir leid, dass Sie wieder Probleme mit dem Collagen-Antikörper ab6311 hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung mit ab86042 für Sie in Auftrag gegeben. Sie hat die Referenznummer xxx und sollte in den nächsten Tagen bei Ihnen ankommen. Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

On your datasheet for ab6311 you state that this antibody has no reactivity with denatured collagen in immunoblots. However, in the enquiries section for that antibody, in question 2, you state that using the positive protein control will yield 3 bands in denaturing conditions. It's unclear to me if this antibody will recognize collagen IV under denatured-reduced conditions. If not, what percentage gel can I use to run a native gel with this antibody?

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Answer

Thank you for your enquiry. I am sorry for the confusion. My colleague was referring to a publication where ab6311 has been applied under denaturing, reduced conditions. However, this antibody was raised to specifically recognizes the native, undenatured form of human collagen type IV. I would therefore recommend that the antibody is applied in this manner. The Collagen group of proteins have many side chains and their migration under denaturing gels is not very predictable. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to measure collagen IV in plasma samples - there are several antibodies you sell. Has any been tested for use in ELISA protocols - as capture and/or detection? My idea was to use ab6586 (coating), ab6311 (detection) and ab7536 (standard). Has this been tested. Can you suggest other combinations? Also, for Brazil, do you send the products directly? What is the means that you send products? Express post? I ask that due to customs regulation here?

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Answer

Thank you for your enquiry. None of our Collagen IV antibodies have been tested together in a sandwich ELISA. They were tested in indirect ELISAs. We ship to Brazil using FedEx International. Please place your order online, or by email, phone or fax (details below). Abcam Ltd 332 Cambridge Science Park Milton Road Cambridge CB4 0FW UK Email:orders@abcam.com Phone:+44 1223 472030 Fax:+44 1223 472038 Office hours: 08.30 - 17.00 hrs (UK) Monday to Friday

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1-10 of 14 Q&A

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