Product nameAnti-Collagen V antibody
See all Collagen V primary antibodies
DescriptionRabbit polyclonal to Collagen V
Specificityab114072 reacts with Collagen V but also exhibits slightly with Collagen IV.
Tested applicationsSuitable for: WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Rat, Human, Common marmoset
Full length native protein purified from Human placenta
After thawing, it can be kept at 2-8°C for more than 3 months.
Storage instructionsShipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. Please see notes section.
Storage bufferConstituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab114072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 184 kDa.|
|IP||Use a concentration of 10 - 30 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Involvement in diseaseEhlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain.
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
This product has been referenced in:
- Miyazaki Y et al. Strategy towards tailored donor tissue-specific pancreatic islet isolation. PLoS One 14:e0216136 (2019). Read more (PubMed: 31075114) »
- Yin H et al. Functionalized thermosensitive hydrogel combined with tendon stem/progenitor cells as injectable cell delivery carrier for tendon tissue engineering. Biomed Mater 13:034107 (2018). Read more (PubMed: 29417934) »