Overview

  • Product name

  • Description

    Rabbit polyclonal to Collagen V
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Indirect ELISA, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Mammals
  • Immunogen

    Collagen Type V purified from human and bovine placenta

  • Positive control

    • Human skin, dermal collagen

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.77% Sodium borate, 0.15% EDTA, 0.43% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/20000 - 1/100000. Use for indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
WB 1/2000 - 1/10000. Predicted molecular weight: 180 kDa. Use native (non-denaturing, non-dissociating) PAGE.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Involvement in disease

    Ehlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • Domain

    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • AB collagen antibody
    • Alpha 1 type V collagen antibody
    • Alpha 2 type V collagen antibody
    • CO5A1_HUMAN antibody
    • COL5A1 antibody
    • Col5A2 antibody
    • COL5A2 protein antibody
    • Col5A3 antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 (V) chain precursor antibody
    • Collagen alpha 2(V) chain antibody
    • Collagen alpha 3(V) chain antibody
    • Collagen alpha-1(V) chain antibody
    • Collagen fetal membrane A polypeptide antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • Collagen type V alpha 3 antibody
    • Collagen V alpha 2 polypeptide antibody
    • CollagenV antibody
    • MGC105115 antibody
    • OTTHUMP00000064637 antibody
    • Pro alpha 1 type V collagen antibody
    • Pro alpha 3(V) collagen antibody
    • Procollagen alpha 2(V) antibody
    • Type V preprocollagen alpha 2 chain antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin, dermal collagen labelling Collagen V with ab134800 at 5 µg/ml, after heat-induced antigen retrieval.

References

ab134800 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134800.
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