Key features and details
- Rabbit polyclonal to Collagen V
- Suitable for: ELISA, ICC/IF, RIA
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Collagen V antibody
See all Collagen V primary antibodies
DescriptionRabbit polyclonal to Collagen V
SpecificityCross-Reactivity (RIA%) Rat Collagen Type V 100% Rat Collagen Types I, III <0.1% Rat Elastin <0.1%
Tested applicationsSuitable for: ELISA, ICC/IF, RIAmore details
Species reactivityReacts with: Rat
Collagen type V extracted and purified from rat skin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferBuffer: Not applicable.
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab21299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen rat tissue sections.
(SP)RIA: Use at an assay dependent dilution.
For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants.
Each laboratory should determine an optimum working titer for use in its particular application.
FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Involvement in diseaseEhlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain.
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab21299 has not yet been referenced specifically in any publications.