Product nameAnti-Collagen V antibody
See all Collagen V primary antibodies
DescriptionRabbit polyclonal to Collagen V
SpecificityThis antibody recognises Collagen Type V and it has negligible cross-reactivity with Type I,II, III, IV, or VI collagens. Non-specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Tested applicationsSuitable for: WB, IP, IHC-Fr, ELISAmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Mammals
Collagen Type V from Human and Bovine placenta.
General notesAntibody concentration is determined by UV absorbance at 280 nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.435% Sodium chloride, 0.146% EDTA, 4.7625% Sodium borate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Our Abpromise guarantee covers the use of ab34714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/50 - 1/200.
IP: Use at an assay dependent dilution.
WB: 1/5000 - 1/10,000. Predicted molecular weight: 180 kDa.
Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Involvement in diseaseEhlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain.
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
ab34714 has been referenced in 1 publication.
- Tamaki T et al. Qualitative alteration of peripheral motor system begins prior to appearance of typical sarcopenia syndrome in middle-aged rats. Front Aging Neurosci 6:296 (2014). PubMed: 25400579