Anti-Collagen V antibody (ab7046)
Key features and details
- Rabbit polyclonal to Collagen V
- Suitable for: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICC
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Collagen V antibody
See all Collagen V primary antibodies -
Description
Rabbit polyclonal to Collagen V -
Host species
Rabbit -
Specificity
Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. -
Tested applications
Suitable for: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICCmore details -
Species reactivity
Reacts with: Mouse, Cow, Human -
Immunogen
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
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General notes
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
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| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
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| IHC-P | (6) |
1/50 - 1/200.
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| IP |
Use at an assay dependent concentration.
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration. PubMed: 18385800
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| Notes |
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WB
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended. |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
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IHC-P
1/50 - 1/200. |
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IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. PubMed: 18385800 |
Target
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Function
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. -
Involvement in disease
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2 -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 282192 Cow
- Entrez Gene: 1289 Human
- Entrez Gene: 1290 Human
- Entrez Gene: 50509 Human
- Entrez Gene: 12831 Mouse
- Entrez Gene: 12832 Mouse
- Entrez Gene: 53867 Mouse
- Omim: 120190 Human
see all -
Alternative names
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
see all
Images
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Western blot - Anti-Collagen V antibody (ab7046)Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab7046 has been referenced in 43 publications.
- Zaccagnini G et al. Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia. Cell Death Dis 12:435 (2021). PubMed: 33934122
- Ding YL et al. COL5A2 as a potential clinical biomarker for gastric cancer and renal metastasis. Medicine (Baltimore) 100:e24561 (2021). PubMed: 33607786
- Berger T et al. NF-?B, iNOS, IL-6, and collagen 1 and 5 expression in healthy and keratoconus corneal fibroblasts after 0.1% riboflavin UV-A illumination. Graefes Arch Clin Exp Ophthalmol 259:1225-1234 (2021). PubMed: 33443628
- Ahmed S & Nowlan NC Initiation and emerging complexity of the collagen network during prenatal skeletal development. Eur Cell Mater 39:136-155 (2020). PubMed: 32103474
- Tsiapalis D et al. The effect of aligned electrospun fibers and macromolecular crowding in tenocyte culture. Methods Cell Biol 157:225-247 (2020). PubMed: 32334716