Overview

  • Product name
    Anti-Collagen V antibody
    See all Collagen V primary antibodies
  • Description
    Rabbit polyclonal to Collagen V
  • Host species
    Rabbit
  • Specificity
    Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applications
    Suitable for: ICC, Sandwich ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Collagen V aa 1-1745.

  • Positive control
    • IHC :Dog skeletal muscle tissue, Human pancreas tissue.
  • General notes

    Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration. PubMed: 18385800
Sandwich ELISA Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).
IHC-Fr 1/50 - 1/200.
IP 1/5000 - 1/10000.
WB 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
IHC-P 1/50 - 1/200.

Target

  • Function
    Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Involvement in disease
    Ehlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • Domain
    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AB collagen antibody
    • Alpha 1 type V collagen antibody
    • Alpha 2 type V collagen antibody
    • CO5A1_HUMAN antibody
    • COL5A1 antibody
    • Col5A2 antibody
    • COL5A2 protein antibody
    • Col5A3 antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 (V) chain precursor antibody
    • Collagen alpha 2(V) chain antibody
    • Collagen alpha 3(V) chain antibody
    • Collagen alpha-1(V) chain antibody
    • Collagen fetal membrane A polypeptide antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • Collagen type V alpha 3 antibody
    • Collagen V alpha 2 polypeptide antibody
    • CollagenV antibody
    • MGC105115 antibody
    • OTTHUMP00000064637 antibody
    • Pro alpha 1 type V collagen antibody
    • Pro alpha 3(V) collagen antibody
    • Procollagen alpha 2(V) antibody
    • Type V preprocollagen alpha 2 chain antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.
    ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Collagen V antibody (ab7046)
    Immunohistochemistry (Frozen sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.

    ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.

    See Abreview

  • Western blot - Anti-Collagen V antibody (ab7046)
    Western blot - Anti-Collagen V antibody (ab7046)
    Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 180 kDa
    Observed band size: 180 kDa
    Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.

References

This product has been referenced in:
  • Steigedal TS  et al. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs. Neoplasia 20:387-400 (2018). WB . Read more (PubMed: 29539586) »
  • Ruibin W  et al. Micro RNA-1298 opposes the effects of chronic oxidative stress on human trabecular meshwork cells via targeting on EIF4E3. Biomed Pharmacother 100:349-357 (2018). WB . Read more (PubMed: 29453044) »

See all 14 Publications for this product

Publishing research using ab7046? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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14 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen V antibody

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Nov 28 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Equus Cell (Tenocyte)
Permeabilization
No
Specification
Tenocyte
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
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Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Permeabilization
No
Specification
Dermal Fibroblast
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
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Adrian Djalali Cuevas

Verified customer

Submitted Sep 25 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester Human Decidua)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
First trimester Human Decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Mar 03 2017

Inquiry: could I use your product (ab27478) as a negative control for ab71310 (I am staining paraffin embeded tissues)?. In your web site you claim that rabbit IgG isotype -ab27478 does not react with any antigen could I use it as a negative control fo...

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Thank you for contacting us with your enquiry.

Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG...

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As a part of my experiment I am comparing breast cancer vs. lymph node metastasis for the expression of: Anti-Collagen V antibody (ab7046) on paraffin embbeded tissues. What positive and negative controls do you recommend to use and how do I obtain them?

Thank you for your inquiry.

For a positive control for ab7046, you can use human pancreas tissue slides as ab4611.

https://www.abcam.com/Pancreas-human-normal-tissue-slides-ab4611.html

For the negative control, you can use...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: .25% · Temperature: 25°C
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Verified customer

Submitted Dec 01 2011

Immunohistochemistry (Frozen sections) abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (skeletal muscle frozen sections)
Specification
skeletal muscle frozen sections
Fixative
Acetone
Permeabilization
No
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Abcam user community

Verified customer

Submitted Sep 20 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen V antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal cortex)
Specification
adrenal cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Sep 12 2007

I used your collagen V for proliferation assay. We incubate 4*10e5 cells/100ul/well with collganen V 10ul + MLR media 90 ul. But the pH of this collagen V sollution (col V10 + MLR media 90) was 5.0, so I nuetralized pH using NaOH to prevent cell damage...

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Thank you for your email. I'm afraid that I do not know how neutralizing the pH in your particular assay is going to affect collagen V. To our knowledge this antibody has not been tested in proliferation assays. I'm sorry that I can't be of further ass...

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