• Product name
  • Description
    Rabbit polyclonal to Collagen V
  • Host species
  • Specificity
    Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applications
    Suitable for: ICC, Sandwich ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Collagen V aa 1-1745.

  • Positive control
    • Natural Collagen V protein (ab7530) can be used as a positive control in WB. IHC :Dog skeletal muscle tissue, Human pancreas tissue.
  • General notes

    Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab7046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration. PubMed: 18385800
Sandwich ELISA Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).
IHC-Fr 1/50 - 1/200.
IP 1/5000 - 1/10000.
WB 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
IHC-P 1/50 - 1/200.


  • Function
    Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Involvement in disease
    Ehlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • Domain
    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AB collagen antibody
    • Alpha 1 type V collagen antibody
    • Alpha 2 type V collagen antibody
    • CO5A1_HUMAN antibody
    • COL5A1 antibody
    • Col5A2 antibody
    • COL5A2 protein antibody
    • Col5A3 antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 (V) chain precursor antibody
    • Collagen alpha 2(V) chain antibody
    • Collagen alpha 3(V) chain antibody
    • Collagen alpha-1(V) chain antibody
    • Collagen fetal membrane A polypeptide antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • Collagen type V alpha 3 antibody
    • Collagen V alpha 2 polypeptide antibody
    • CollagenV antibody
    • MGC105115 antibody
    • OTTHUMP00000064637 antibody
    • Pro alpha 1 type V collagen antibody
    • Pro alpha 3(V) collagen antibody
    • Procollagen alpha 2(V) antibody
    • Type V preprocollagen alpha 2 chain antibody
    see all


  • Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 180 kDa
    Observed band size: 180 kDa
    Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes
  • ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.

    See Abreview

  • ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.


This product has been referenced in:
  • Steigedal TS  et al. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs. Neoplasia 20:387-400 (2018). WB . Read more (PubMed: 29539586) »
  • Ruibin W  et al. Micro RNA-1298 opposes the effects of chronic oxidative stress on human trabecular meshwork cells via targeting on EIF4E3. Biomed Pharmacother 100:349-357 (2018). WB . Read more (PubMed: 29453044) »
See all 19 Publications for this product

Customer reviews and Q&As

11-15 of 15 Abreviews or Q&A


Thank you for your email. I'm afraid that I do not know how neutralizing the pH in your particular assay is going to affect collagen V. To our knowledge this antibody has not been tested in proliferation assays. I'm sorry that I can't be of further assistance regarding your inquiry.

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You want to add glycerol to 50% before freezing. So what your labmate did (vial is 50% antibody, 50% glycerol) is correct.

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Thank you for your patience in awaiting a response. Collagen V is over 400kDa.It is important to note that this product will not be supported in reduced conditions. Please use this product in non-reducing conditions. The public-facing data sheet. Thank you for bringing this matter to my attention.

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The immunogen used to generate Rabbit polyclonal to Collagen V was Collagen Type V from adult human and bovine tissues. As the antibody is a polyclonal and was raised agaisnt the whole collagen molecule it will recognise a number of epitopes on the surface of the collagen molecule.

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11-15 of 15 Abreviews or Q&A

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