Product nameAnti-Collagen V antibody
See all Collagen V primary antibodies
DescriptionRabbit polyclonal to Collagen V
SpecificityNegligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Tested applicationsSuitable for: ICC, Sandwich ELISA, IHC-Fr, IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Cow, Human
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
- Natural Collagen V protein (ab7530) can be used as a positive control in WB. IHC :Dog skeletal muscle tissue, Human pancreas tissue.
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Our Abpromise guarantee covers the use of ab7046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration. PubMed: 18385800|
|Sandwich ELISA||Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).|
|IHC-Fr||1/50 - 1/200.|
|IP||1/5000 - 1/10000.|
|WB||1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.|
|IHC-P||1/50 - 1/200.|
FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Involvement in diseaseEhlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain.
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.
ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
This product has been referenced in:
- Gaspar D et al. Multifactorial bottom-up bioengineering approaches for the development of living tissue substitutes. FASEB J 33:5741-5754 (2019). Read more (PubMed: 30681885) »
- Steigedal TS et al. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs. Neoplasia 20:387-400 (2018). WB . Read more (PubMed: 29539586) »