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Inquiry: could I use your product (ab27478) as a negative control for ab71310 (I am staining paraffin embeded tissues)?. In your web site you claim that rabbit IgG isotype -ab27478 does not react with any antigen could I use it as a negative control for ab100810 and ab7046 as well?
Asked on Sep 21 2012
Thank you for contacting us with your enquiry.
Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG molecules in ab27478 were collected from rabbit serum prior to any immunization, there is no specificity for any particular antigens.
I hope that this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.
Answered on Sep 21 2012