Recombinant Anti-Collagen V antibody [EPR23762-54] (ab275881)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23762-54] to Collagen V
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Collagen V antibody [EPR23762-54]
See all Collagen V primary antibodies -
Description
Rabbit monoclonal [EPR23762-54] to Collagen V -
Host species
Rabbit -
Specificity
The immunogen of this antibody is based on Collagen V-COL5A2, low homology with Collagen V-COL5A1 based on immunogen design.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse articular cartilage tissue lysate; Rat and Mouse articular cartilage organ culture supernatant.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23762-54 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 32, 36, 145 kDa (predicted molecular weight: 184 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 32, 36, 145 kDa (predicted molecular weight: 184 kDa). |
Target
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Function
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. -
Involvement in disease
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2 -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1289 Human
- Entrez Gene: 1290 Human
- Entrez Gene: 50509 Human
- Entrez Gene: 12831 Mouse
- Entrez Gene: 12832 Mouse
- Entrez Gene: 53867 Mouse
- Entrez Gene: 60379 Rat
- Entrez Gene: 85250 Rat
see all -
Alternative names
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
see all
Images
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All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Mouse articular cartilage organ culture supernatant at 10 µl
Lane 2 : Mouse articular cartilage organ culture supernatant treated with PNGase F at 10 µl
Lane 3 : Mouse articular cartilage tissue lysate at 20 µg
Lane 4 : Mouse articular cartilage tissue lysate treated with PNGase F at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 32,36 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20625483).
Glycosylation leads to the shift of the bands.
Exposure time: 6 seconds.
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All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Rat placenta organ culture supernatant at 30 µl
Lane 2 : Rat articular cartilage tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 145,32 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 23262228, 20625483).
Exposure time: Lane 1: 92 seconds Lane 2: 1 seconds.
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All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Mouse articular cartilage tissue lysate
Lane 2 : Mouse PBMCs (peripheral blood mononuclear cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20625483).
Low expression: Mouse PBMCs (database:HPA).
Exposure time: Lane 1: 5 seconds Lane 2: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab275881 has been referenced in 1 publication.
- Kucherenko MM et al. Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease. Nat Commun 14:4416 (2023). PubMed: 37479718