Anti-Collagen VI antibody (ab6588)
- Datasheet
- References (76)
- Protocols
Overview
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Product name
Anti-Collagen VI antibody
See all Collagen VI primary antibodies -
Description
Rabbit polyclonal to Collagen VI -
Host species
Rabbit -
Specificity
Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. -
Tested applications
Suitable for: ICC/IF, ELISA, IHC-Fr, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig
Predicted to work with: Mammals
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Immunogen
Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
Database link: P12109 -
Positive control
- WB: HepG2 whole cell lysate. IHC-P: Human placenta and testis tissue.
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General notes
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.01% Sodium azide
Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab6588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250 - 1/500. | |
| ELISA | 1/4000 - 1/8000. | |
| IHC-Fr | 1/50 - 1/200. | |
| IP | 1/100. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa). This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
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| IHC-P | Use at an assay dependent concentration. PubMed: 18065394 |
Target
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Function
Collagen VI acts as a cell-binding protein. -
Involvement in disease
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
Sequence similarities
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1291 Human
- Entrez Gene: 1292 Human
- Entrez Gene: 1293 Human
- Entrez Gene: 12833 Mouse
- Entrez Gene: 12834 Mouse
- Entrez Gene: 12835 Mouse
- Entrez Gene: 294337 Rat
- Entrez Gene: 361821 Rat
see all -
Alternative names
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Collagen VI antibody (ab6588)Szatkowski et al PLoS One. 2013 Dec 4;8(12):e81175. doi: 10.1371/journal.pone.0081175. eCollection 2013. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Abdominal obesity in PKR1ad−/− mice is due to preadipocyte proliferation and differentiation.
D) Macrophage specific CD68 staining and quantification of macrophages on cryosectioned adipose tissues derived from PKR1ad−/− and L2/L2 mice (*p<0.05, n = 20 HPF, ×40 objective).
E) Illustration and histogram shows the PECAM-1 staining and capillary formation changes between the adipose tissues of PKR1ad−/− and L2/L2 mice (*p<0.05, n = 20 HPF, ×40 objective).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody (ab6588)Stribos et al PLoS One. 2017 Apr 21;12(4):e0175898. doi: 10.1371/journal.pone.0175898. eCollection 2017. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Representative images of collagen type VI staining in healthy and fibrotic renal tissue.
Magnification 200x for collagen VI staining (ab6588) and 100x for PSR staining. PSR, Picro-Sirius Red.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody (ab6588)Image is courtesy of an anonymous AbReview.Immunohistochemical analysis of 10% buffered formalin-fixed swine lung tissue sections, labelling Collagen VI with ab6588 at a dilution of 1/400 incubated for 12 hours at 4°C. Antigen retrival was with 10mM sodium citrate buffer pH 6.0 (heat mediated). Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was an undiluted Donkey anti-Rabbit polyclonal Alexa Fluor® 647 conjugate.
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Western blot - Anti-Collagen VI antibody (ab6588)Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 108 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody (ab6588)This image is courtesy of an Abreview submitted by Vladimir Milenkovicab6588 staining (green) Collagen VI in a primary cell culture from mouse retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/500 (1% goat serum, 0.1% Triton X-100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG (ab150077), dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody (ab6588)Image courtesy of an anonymous Abreview.ab6588 staining Collagen VI in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 30 minutes at 20°C and then incubated with ab6588 at a 1/50 dilution for 1 hour at 37°C. The secondary used was an Alexa-Fluor® 555 conjugated goat anti-rabbit polyclonal (ab150078), used at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody (ab6588)This image is courtesy of an Abreview submitted by Vladimir Milenkovicab6588 staining Collagen VI in a primary cell culture from pig retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/250 (1% goat serum, 0.1% Triton X-100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody (ab6588)ab6588 at 1/400 dilution staining Collagen VI in human placenta (Top) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody (ab6588)Image courtesy of an anonymous Abreview.Immunohistochemical analysis of formaldehyde fixed human colon tissue sections labeling Collagen VI with ab6588 at a dilution of 1/200. The secondary antibody used was HRP conjugated rabbit polyclonal secondary. Antigen retrieval was heat mediated.
Protocols
References
This product has been referenced in:
- Gaspar D et al. Multifactorial bottom-up bioengineering approaches for the development of living tissue substitutes. FASEB J 33:5741-5754 (2019). Read more (PubMed: 30681885) »
- Nagy JA et al. Permittivity of ex vivo healthy and diseased murine skeletal muscle from 10?kHz to 1?MHz. Sci Data 6:37 (2019). Read more (PubMed: 31000708) »
