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    collagen-vi-antibody-ab6588.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen VI antibody (ab6588)

  • Datasheet
  • SDS
Reviews (21)Q&A (28)References (96)

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Western blot - Anti-Collagen VI antibody (ab6588)

    Key features and details

    • Rabbit polyclonal to Collagen VI
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Collagen VI antibody [EPR17072] (ab182744)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Collagen VI antibody
      See all Collagen VI primary antibodies
    • Description

      Rabbit polyclonal to Collagen VI
    • Host species

      Rabbit
    • Specificity

      Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals
    • Immunogen

      Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
      Database link: P12109

    • Positive control

      • WB: HepG2 whole cell lysate.
    • General notes

      At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Collagen
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Native Cow Collagen VI protein (ab7531)
      • Native Human Collagen VI protein (ab7538)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6588 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).

    This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

    Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).

    This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

    Target

    • Function

      Collagen VI acts as a cell-binding protein.
    • Involvement in disease

      Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
      Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
    • Sequence similarities

      Belongs to the type VI collagen family.
      Contains 3 VWFA domains.
    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P12109 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1291 Human
      • Entrez Gene: 1292 Human
      • Entrez Gene: 1293 Human
      • Entrez Gene: 12833 Mouse
      • Entrez Gene: 12834 Mouse
      • Entrez Gene: 12835 Mouse
      • Entrez Gene: 294337 Rat
      • Entrez Gene: 361821 Rat
      • Omim: 120220 Human
      • Omim: 120240 Human
      • Omim: 120250 Human
      • SwissProt: P12109 Human
      • SwissProt: P12110 Human
      • SwissProt: P12111 Human
      • SwissProt: Q02788 Mouse
      • SwissProt: Q04857 Mouse
      • Unigene: 474053 Human
      • Unigene: 2509 Mouse
      see all
    • Alternative names

      • Alpha 1 (VI) chain (61 AA) antibody
      • CO6A1_HUMAN antibody
      • COL6A1 antibody
      • COL6A2 antibody
      • COL6A3 antibody
      • collagen 6 antibody
      • Collagen alpha 2(VI) chain antibody
      • Collagen alpha 3(VI) chain antibody
      • Collagen alpha-1(VI) chain antibody
      • collagen six antibody
      • Collagen type VI alpha 1 antibody
      • Collagen type VI alpha 2 antibody
      • Collagen type VI alpha 3 antibody
      • Collagen VI alpha 1 polypeptide antibody
      • Collagen VI alpha 2 polypeptide antibody
      • Collagen VI alpha 3 polypeptide antibody
      • CollagenVI antibody
      • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
      • OPLL antibody
      • PP3610 antibody
      see all

    Images

    • Western blot - Anti-Collagen VI antibody (ab6588)
      Western blot - Anti-Collagen VI antibody (ab6588)
      Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 108 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (96)

    Publishing research using ab6588? Please let us know so that we can cite the reference in this datasheet.

    ab6588 has been referenced in 96 publications.

    • Jones JEC  et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
    • Harish P  et al. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease. Front Physiol 11:184 (2020). PubMed: 32194441
    • Semple C  et al. Partial Weight-Bearing in Female Rats: Proof of Concept in a Martian-Gravity Analog. Front Physiol 11:302 (2020). PubMed: 32308630
    • Bolduc V  et al. Pathogenic variants in COL6A3 cause Ullrich-like congenital muscular dystrophy in young Labrador Retriever dogs. Neuromuscul Disord 30:360-367 (2020). PubMed: 32439203
    • Farini A  et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 49 Abreviews or Q&A

    Question

    Thanks for your kindly reply, after contact with this customer, she would like to have a credit note,

    could you please help her to create a credit note ?



    I deeply appreciate your great assistance!



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Thank you for contacting us.

    Your credit note ID is 22524.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Equus Cell (Tenocyte)
    Permeabilization
    No
    Specification
    Tenocyte
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Adrian Djalali Cuevas

    Verified customer

    Submitted Oct 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Dermal Fibroblast)
    Permeabilization
    No
    Specification
    Dermal Fibroblast
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Adrian Djalali Cuevas

    Verified customer

    Submitted Sep 26 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue (Cardiac tissue)
    Permeabilization
    No
    Specification
    Cardiac tissue
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 31 2017

    IHC - Wholemount abreview for Anti-Collagen VI antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (intestine organoids)
    Specification
    intestine organoids
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (adipocyte)
    Permeabilization
    Yes - 0.05% TX-100
    Specification
    adipocyte
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    Artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 07 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 01 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Specification
    colon
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 20 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (artery)
    Permeabilization
    No
    Specification
    artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 29 2016

    1-10 of 49 Abreviews or Q&A

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