Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen VI
  • Host species
    Rabbit
  • Specificity
    Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig
    Predicted to work with: Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
    Database link: P12109

  • Positive control
    • Natural Cow Collagen VI protein (ab7531) can be used as a positive control in WB. HepG2. Pig retinal pigment epithelium. Human placenta.
  • General notes

    At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
ELISA 1/4000 - 1/8000.
IHC-Fr 1/50 - 1/200.
IP Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa). This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
IHC-P Use at an assay dependent concentration. PubMed: 18065394

Target

  • Function
    Collagen VI acts as a cell-binding protein.
  • Involvement in disease
    Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • Sequence similarities
    Belongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • COL6A2 antibody
    • COL6A3 antibody
    • Collagen alpha 2(VI) chain antibody
    • Collagen alpha 3(VI) chain antibody
    • Collagen alpha-1(VI) chain antibody
    • Collagen type VI alpha 1 antibody
    • Collagen type VI alpha 2 antibody
    • Collagen type VI alpha 3 antibody
    • Collagen VI alpha 1 polypeptide antibody
    • Collagen VI alpha 2 polypeptide antibody
    • Collagen VI alpha 3 polypeptide antibody
    • CollagenVI antibody
    • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
    • OPLL antibody
    • PP3610 antibody
    see all

Images

  • Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 108 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of 10% buffered formalin-fixed swine lung tissue sections, labelling Collagen VI with ab6588 at a dilution of 1/400 incubated for 12 hours at 4°C. Antigen retrival was with 10mM sodium citrate buffer pH 6.0 (heat mediated). Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 647 conjugtate undiluted.

    See Abreview

  • ab6588 staining (green) Collagen VI in a primary cell culture from Mouse retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/500 (1% goat serum, 0.1% TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG (ab150077), dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • ab6588 staining Collagen VI in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 30 minutes at 20°C and then incubated with ab6588 at a 1/50 dilution for 1 hour at 37°C. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal (ab150078), used at a 1/500 dilution.

    See Abreview

  • ab6588 staining Collagen VI in a primary cell culture from Pig retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/250 (1% goat serum, 0.1% TX100, 1XPBS)  for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • ab6588 at 1/400 dilution staining Collagen VI in Human placenta (Top) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

  • Immunohistochemical analysis of Formaldehyde fixed human colon tissue sections labelling Collagen VI with ab6588 at a dilution of 1/200. The secondary antibody used was HRP conjugated rabbit polyclonal secondary. Antigen retrieval was heat mediated. 

    See Abreview

References

This product has been referenced in:
  • Munro ML  et al. Highly variable contractile performance correlates with myocyte content in trabeculae from failing human hearts. Sci Rep 8:2957 (2018). Read more (PubMed: 29440728) »
  • Windhövel C  et al. Comparison of Six Different Silicones In Vitro for Application as Glaucoma Drainage Device. Materials (Basel) 11:N/A (2018). ICC/IF ; Human . Read more (PubMed: 29495462) »
See all 52 Publications for this product

Customer reviews and Q&As

1-10 of 48 Abreviews or Q&A

Answer

Thank you for contacting us.

Your credit note ID is 22524.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Equus Cell (Tenocyte)
Permeabilization
No
Specification
Tenocyte
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Permeabilization
No
Specification
Dermal Fibroblast
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Adrian Djalali Cuevas

Verified customer

Submitted Sep 26 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue (Cardiac tissue)
Permeabilization
No
Specification
Cardiac tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 31 2017

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids

Abcam user community

Verified customer

Submitted Mar 29 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (adipocyte)
Permeabilization
Yes - 0.05% TX-100
Specification
adipocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Sep 07 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Sep 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (artery)
Permeabilization
No
Specification
artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 29 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Jan 28 2016

1-10 of 48 Abreviews or Q&A

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