Recombinant Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-334417-anti-collagen-vi-antibody-epr17077-c-terminal-western-blot.jpg "Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17077] to Collagen VI - C-terminal
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Collagen VI antibody [EPR17077] - C-terminal
See all Collagen VI primary antibodies -
Description
Rabbit monoclonal [EPR17077] to Collagen VI - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart, skeletal muscle and WI-38 (Human lung) whole cell lysate. Mouse heart, kidney and spleen. Rat heart, kidney and spleen. NIH/3T3 whole cell lysate. IHC-P: Human colon and Mouse cardiac muscle tissue. ICC/IF: NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17077 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 147 kDa (predicted molecular weight: 109 kDa).
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| Notes |
|---|
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IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 147 kDa (predicted molecular weight: 109 kDa). |
Target
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Function
Collagen VI acts as a cell-binding protein. -
Involvement in disease
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
Sequence similarities
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1291 Human
- Entrez Gene: 1292 Human
- Entrez Gene: 1293 Human
- Entrez Gene: 12833 Mouse
- Entrez Gene: 12834 Mouse
- Entrez Gene: 12835 Mouse
- Entrez Gene: 294337 Rat
- Entrez Gene: 361821 Rat
see all -
Alternative names
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
see all
Images
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Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)All lanes : Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T whole cell lysate
Lane 2 : COL6A1 knockout HEK-293T whole cell lysate
Lane 3 : Human Skeletal Muscle whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 109 kDaLanes 1 - 3: Merged signal (red and green). Green - ab199720 observed at 109 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab199720 was shown to recognize Collagen VI in wild-type Hek 293T cells as signal was lost at the expected MW in COL6A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and COL6A1 knockout samples were subjected to SDS-PAGE. Ab199720 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-242316-anti-collagen-vi-antibody-epr17077-c-terminal-western-blot.jpg)
Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)All lanes : Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/10000 dilution
Lane 1 : Human fetal heart
Lane 2 : Human skeletal muscle
Lane 3 : WI-38 (Human lung) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 147 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-242314-anti-collagen-vi-antibody-epr17077-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on stromal cells of Human colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-242315-anti-collagen-vi-antibody-epr17077-c-terminal-western-blot.jpg)
Western blot - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)All lanes : Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720) at 1/1000 dilution
Lane 1 : Mouse heart
Lane 2 : Mouse kidney
Lane 3 : Mouse spleen
Lane 4 : Rat heart
Lane 5 : Rat kidney
Lane 6 : Rat spleen
Lane 7 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 147 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the following literature: PMID:16130093 and 21186846
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-242313-anti-collagen-vi-antibody-epr17077-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Collagen VI with ab199720 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Extracellular matrix staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)](https://www.abcam.com/ps/products/199/ab199720/Images/ab199720-8-benefits-of-recombinant-antibodies.png)
Anti-Collagen VI antibody [EPR17077] - C-terminal (ab199720)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab199720 has been referenced in 4 publications.
- Wishart AL et al. Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis. Sci Adv 6:N/A (2020). PubMed: 33087348
- Chen PH et al. High-Temperature Requirement A1 Protease as a Rate-Limiting Factor in the Development of Osteoarthritis. Am J Pathol 189:1423-1434 (2019). PubMed: 31051168
- Wang D et al. Insulin-like growth factor-1 engaged in the mandibular condylar cartilage degeneration induced by experimental unilateral anterior crossbite. Arch Oral Biol 98:17-25 (2019). PubMed: 30419485
- Endicott J et al. Authentication of collagen VI antibodies. BMC Res Notes 10:358 (2017). PubMed: 28755659
