Recombinant Anti-Collagen VI antibody [EPR7888(N)] (ab172606)
![Western blot - Anti-Collagen VI antibody [EPR7888(N)] (ab172606)](https://www.abcam.com/ps/products/172/ab172606/Images/ab172606-195189-anti-collagen-vi-antibody-epr7888n-western-blot.jpg "Western blot - Anti-Collagen VI antibody [EPR7888(N)] (ab172606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7888(N)] to Collagen VI
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Collagen VI antibody [EPR7888(N)]
See all Collagen VI primary antibodies -
Description
Rabbit monoclonal [EPR7888(N)] to Collagen VI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human Collagen VI aa 200-300. The exact sequence is proprietary.
Database link: P12110 -
Positive control
- Human skin, placenta and fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7888(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Mouse
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 109 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 109 kDa). |
Target
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Function
Collagen VI acts as a cell-binding protein. -
Involvement in disease
Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
Sequence similarities
Belongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1291 Human
- Entrez Gene: 1292 Human
- Entrez Gene: 1293 Human
- Entrez Gene: 12833 Mouse
- Entrez Gene: 12834 Mouse
- Entrez Gene: 12835 Mouse
- Entrez Gene: 294337 Rat
- Entrez Gene: 361821 Rat
see all -
Alternative names
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
see all
Images
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Western blot - Anti-Collagen VI antibody [EPR7888(N)] (ab172606)All lanes : Anti-Collagen VI antibody [EPR7888(N)] (ab172606) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human placenta lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 109 kDa -
![Anti-Collagen VI antibody [EPR7888(N)] (ab172606)](https://www.abcam.com/ps/products/172/ab172606/Images/ab172606-2-benefits-of-recombinant-antibodies.png)
Anti-Collagen VI antibody [EPR7888(N)] (ab172606)
Datasheets and documents
References (2)
ab172606 has been referenced in 2 publications.
- Endicott J et al. Authentication of collagen VI antibodies. BMC Res Notes 10:358 (2017). PubMed: 28755659
- Safaee H et al. Tethered Jagged-1 Synergizes with Culture Substrate Stiffness to Modulate Notch-Induced Myogenic Progenitor Differentiation. Cell Mol Bioeng 10:501-513 (2017). PubMed: 31719873
