Recombinant
Anti-Collagen VI antibody [EPR7888(N)] (ab172606)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-Collagen VI antibody [EPR7888(N)]
See all Collagen VI primary antibodies -
DescriptionRabbit monoclonal [EPR7888(N)] to Collagen VI
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human Collagen VI aa 200-300. The exact sequence is proprietary.
Database link: P12110 -
Positive control
- Human skin, placenta and fetal heart lysates.
-
General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR7888(N)
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab172606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 109 kDa). |
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
-
FunctionCollagen VI acts as a cell-binding protein.
-
Involvement in diseaseDefects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. -
Sequence similaritiesBelongs to the type VI collagen family.
Contains 3 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
-
Database links
- Entrez Gene: 1291 Human
- Entrez Gene: 1292 Human
- Entrez Gene: 1293 Human
- Entrez Gene: 12833 Mouse
- Entrez Gene: 12834 Mouse
- Entrez Gene: 12835 Mouse
- Entrez Gene: 294337 Rat
- Entrez Gene: 361821 Rat
see all -
Alternative names
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
see all
Images
-
![Western blot - Anti-Collagen VI antibody [EPR7888(N)] (ab172606)](https://www.abcam.com/ps/products/172/ab172606/Images/ab172606-195189-ab172606.jpg)
Western blot - Anti-Collagen VI antibody [EPR7888(N)] (ab172606)All lanes : Anti-Collagen VI antibody [EPR7888(N)] (ab172606) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human placenta lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 109 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Endicott J et al. Authentication of collagen VI antibodies. BMC Res Notes 10:358 (2017). Read more (PubMed: 28755659) »
