Key features and details
- Goat polyclonal to Collagen VII
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Collagen VII antibody
See all Collagen VII primary antibodies
DescriptionGoat polyclonal to Collagen VII
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
- WB: Rat testis lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223639 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1.5 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 295 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionStratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Involvement in diseaseEpidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
Epidermolysis bullosa dystrophica, autosomal dominant
Epidermolysis bullosa dystrophica, autosomal recessive
Epidermolysis bullosa dystrophica, Pasini type
Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
Transient bullous dermolysis of the newborn
Epidermolysis bullosa dystrophica, pretibial type
Epidermolysis bullosa dystrophica, Bart type
Epidermolysis bullosa pruriginosa
Nail disorder, non-syndromic congenital, 8
Epidermolysis bullosa dystrophica, with subcorneal cleavage
Sequence similaritiesContains 1 BPTI/Kunitz inhibitor domain.
Contains 9 fibronectin type-III domains.
Contains 2 VWFA domains.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Alpha 1 type VII collagen antibody
- CO7A1_HUMAN antibody
- Col7a1 antibody
Anti-Collagen VII antibody (ab223639) at 0.5 µg/ml + Rat testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 295 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223639 has not yet been referenced specifically in any publications.