Overview

  • Product name

    Anti-Collagen VII antibody
    See all Collagen VII primary antibodies
  • Description

    Goat polyclonal to Collagen VII
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Collagen VII aa 81-92 (internal sequence) (Cysteine residue). (NP_000085.1).
    Sequence:

    C-TVQYSDDPRTEF


    Database link: Q02388

  • Positive control

    • WB: Rat testis lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223639 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1.5 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 295 kDa).

Target

  • Function

    Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
  • Involvement in disease

    Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
    Epidermolysis bullosa dystrophica, autosomal dominant
    Epidermolysis bullosa dystrophica, autosomal recessive
    Epidermolysis bullosa dystrophica, Pasini type
    Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
    Transient bullous dermolysis of the newborn
    Epidermolysis bullosa dystrophica, pretibial type
    Epidermolysis bullosa dystrophica, Bart type
    Epidermolysis bullosa pruriginosa
    Nail disorder, non-syndromic congenital, 8
    Epidermolysis bullosa dystrophica, with subcorneal cleavage
  • Sequence similarities

    Contains 1 BPTI/Kunitz inhibitor domain.
    Contains 9 fibronectin type-III domains.
    Contains 2 VWFA domains.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha 1 type VII collagen antibody
    • CO7A1_HUMAN antibody
    • Col7a1 antibody
    • Collagen 7 antibody
    • Collagen alpha 1(VII) chain antibody
    • Collagen alpha-1(VII) chain antibody
    • Collagen type VII alpha 1 antibody
    • Collagen VII alpha 1 polypeptide antibody
    • CollagenVII antibody
    • EBD 1 antibody
    • EBD1 antibody
    • EBDCT antibody
    • EBR 1 antibody
    • EBR1 antibody
    • LC collagen antibody
    • Long chain collagen antibody
    • Long-chain collagen antibody
    see all

Images

  • Anti-Collagen VII antibody (ab223639) at 0.5 µg/ml + Rat testis lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 295 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223639 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223639.
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