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Full length native protein (purified) corresponding to Human Collagen VII.
Our Abpromise guarantee covers the use of ab93350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.
Immunohistochemical staining of human skin tissue (frozen sections) labeling Collagen VII with ab93350. Samples were fixed using acetone and 3% BSA was used as blocking agent for 1 hour at room temperature. Samples were incubated with ab93350 for 1 hour at 1/200 dilution. An Alexa Fluor-488 rabbit polyclonal was used as a secondary antibody.
Immunofluorescence staining of Collagen VII in normal human keratinocytes. Second antibody for staining of cells: goat-anti-rabbit IgG Alexa 488
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