Product nameAnti-Collagen VII antibody
See all Collagen VII primary antibodies
DescriptionRabbit polyclonal to Collagen VII
Tested applicationsSuitable for: WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Human, Common marmoset
Full length native protein (purified) corresponding to Human Collagen VII.
- Human skin tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab93350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EIA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
IP: Use at a concentration of 2.5 - 10 µg/ml.
WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionStratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Involvement in diseaseEpidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
Epidermolysis bullosa dystrophica, autosomal dominant
Epidermolysis bullosa dystrophica, autosomal recessive
Epidermolysis bullosa dystrophica, Pasini type
Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
Transient bullous dermolysis of the newborn
Epidermolysis bullosa dystrophica, pretibial type
Epidermolysis bullosa dystrophica, Bart type
Epidermolysis bullosa pruriginosa
Nail disorder, non-syndromic congenital, 8
Epidermolysis bullosa dystrophica, with subcorneal cleavage
Sequence similaritiesContains 1 BPTI/Kunitz inhibitor domain.
Contains 9 fibronectin type-III domains.
Contains 2 VWFA domains.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Alpha 1 type VII collagen antibody
- CO7A1_HUMAN antibody
- Col7a1 antibody
Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.
Immunohistochemical staining of human skin tissue (frozen sections) labeling Collagen VII with ab93350. Samples were fixed using acetone and 3% BSA was used as blocking agent for 1 hour at room temperature. Samples were incubated with ab93350 for 1 hour at 1/200 dilution. An Alexa Fluor-488 rabbit polyclonal was used as a secondary antibody.
Immunofluorescence staining of Collagen VII in normal human keratinocytes. Second antibody for staining of cells: goat-anti-rabbit IgG Alexa 488
This product has been referenced in:
- Twaroski K et al. Revertant Mosaic Fibroblasts in Recessive Dystrophic Epidermolysis Bullosa. Br J Dermatol N/A:N/A (2019). Read more (PubMed: 30924923) »
- Russo V et al. Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Sci Rep 8:9690 (2018). Read more (PubMed: 29946113) »