Overview

  • Product name

    Anti-Collagen VII antibody
    See all Collagen VII primary antibodies
  • Description

    Rabbit polyclonal to Collagen VII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Common marmoset
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen VII.

  • Positive control

    • Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab93350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
Flow Cyt
ICC/IF
IHC-Fr
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    EIA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
    IP: Use at a concentration of 2.5 - 10 µg/ml.
    WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • Involvement in disease

      Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • Sequence similarities

      Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen 7 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all

    Images

    • Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.

      See Abreview

    • Immunohistochemical staining of human skin tissue (frozen sections) labeling Collagen VII with ab93350. Samples were fixed using acetone and 3% BSA was used as blocking agent for 1 hour at room temperature. Samples were incubated with ab93350 for 1 hour at 1/200 dilution. An Alexa Fluor-488 rabbit polyclonal was used as a secondary antibody.

      See Abreview

    • Immunofluorescence staining of Collagen VII in normal human keratinocytes. Second antibody for staining of cells: goat-anti-rabbit IgG Alexa 488

    References

    This product has been referenced in:

    • Twaroski K  et al. Revertant Mosaic Fibroblasts in Recessive Dystrophic Epidermolysis Bullosa. Br J Dermatol N/A:N/A (2019). Read more (PubMed: 30924923) »
    • Russo V  et al. Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Sci Rep 8:9690 (2018). Read more (PubMed: 29946113) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Jul 12 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    No
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 19 2014

    Answer

    Thank you for your inquiry.

    I heard back from the lab that our anti-Collagen VII antibody is specific only for helical domains (collagen VII fragments). The non-collagen fragments obtained after pepsin digestion are broken and cannot be detected.

    I'm not sure if any of our Collagen VII antibodies will suit your needs, but take a look at ab80790 as this one has quite a few references and has been used for cell staining.
    https://www.abcam.com/Collagen-VII-antibody-LH7-2-BSA-and-Azide-free-ab80790.html
    I hope this information helps. Please contact us with any other questions.

    Read More

    Question
    Answer

    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for your enquiry.

    I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are none to provide on this occasion for ab93350 and ab96885.

    Should you have any further questions, please do not hesitate to contact us.

    Read More

    Question
    Answer

    Thank you for contacting us. The BLAST results show 84% homology between the full-length human (immunogen) and mouse and is likely to work. If you are interested in this product we do have a testing discount program which acts as a buy one get one free, where you receive a code good for a free antibody after you submit a review for your mouse data on our website. Please let me know if you would like to participate in this program. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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