Anti-Collagen VII antibody - BSA and Azide free (ab93350)
Key features and details
- Rabbit polyclonal to Collagen VII - BSA and Azide free
- Suitable for: ICC/IF
- Reacts with: Human, Common marmoset
- Isotype: IgG
Overview
-
Product name
Anti-Collagen VII antibody - BSA and Azide free
See all Collagen VII primary antibodies -
Description
Rabbit polyclonal to Collagen VII - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human, Common marmoset -
Immunogen
Full length native protein (purified) corresponding to Human Collagen VII.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Notes |
---|
EIA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
IP: Use at a concentration of 2.5 - 10 µg/ml.
WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. -
Involvement in disease
Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
Epidermolysis bullosa dystrophica, autosomal dominant
Epidermolysis bullosa dystrophica, autosomal recessive
Epidermolysis bullosa dystrophica, Pasini type
Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
Transient bullous dermolysis of the newborn
Epidermolysis bullosa dystrophica, pretibial type
Epidermolysis bullosa dystrophica, Bart type
Epidermolysis bullosa pruriginosa
Nail disorder, non-syndromic congenital, 8
Epidermolysis bullosa dystrophica, with subcorneal cleavage -
Sequence similarities
Contains 1 BPTI/Kunitz inhibitor domain.
Contains 9 fibronectin type-III domains.
Contains 2 VWFA domains. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1294 Human
- Omim: 120120 Human
- SwissProt: Q02388 Human
- Unigene: 476218 Human
-
Alternative names
- Alpha 1 type VII collagen antibody
- CO7A1_HUMAN antibody
- Col7a1 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab93350 has been referenced in 14 publications.
- Yokota M et al. Trehangelins ameliorate inflammation-induced skin senescence by suppressing the epidermal YAP-CCN1 axis. Sci Rep 12:952 (2022). PubMed: 35046484
- Witt J et al. Cryopreservation in a Standard Freezer: -28 °C as Alternative Storage Temperature for Amniotic Membrane Transplantation. J Clin Med 11:N/A (2022). PubMed: 35207382
- Thompson EL et al. Genes and compounds that increase type VII collagen expression as potential treatments for dystrophic epidermolysis bullosa. Exp Dermatol 31:1065-1075 (2022). PubMed: 35243691
- Evtushenko NA et al. hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination. Int J Mol Sci 22:N/A (2021). PubMed: 33916959
- Beilin AK et al. Signatures of Dermal Fibroblasts from RDEB Pediatric Patients. Int J Mol Sci 22:N/A (2021). PubMed: 33670258